Transcriptional REGUlation as a mediator of bacterial interactions in the microB...
Bacteria from the gut microbiota constantly interact with a range of abiotic and biotic factors. Perturbations in these factors, such as nutrient depletion or exposure to exogenous microbes, require specific bacterial responses th...
Bacteria from the gut microbiota constantly interact with a range of abiotic and biotic factors. Perturbations in these factors, such as nutrient depletion or exposure to exogenous microbes, require specific bacterial responses that involve tightly controlled gene expression changes. However, the transcriptional regulation mechanisms promoting such gene expression changes remain poorly understood despite their importance.
REGUBIOME will characterize how responses to specific biotic and abiotic stimuli are regulated at the transcriptional level in two health-associated gut bacteria: one probiotic (Lacticaseibacillus rhamnosus) and one pathobiont (Fusobacterium nucleatum, previously linked to colorectal cancer). This project relies on combining experimental platforms of increasing complexity to systematically characterize the responses of these two bacteria to defined biotic and abiotic factors, and to evaluate how these responses impact host health. First, I will develop a novel high-throughput functional screening tool, coupling CRISPR interference, microfluidic platforms for single-cell isolation, and transcriptomic profiling of isolates. By applying this tool to in vitro monocultures, I will reconstruct the first-ever gene regulatory networks in these bacteria and identify mechanisms of response to different abiotic factors. Second, microfluidic co-culturing of these two strains with multiple other bacteria, coupled with metatranscriptomic profiling, will reveal the regulatory mechanisms mediating the interactions with other microbiome members. Lastly, in vivo work in mouse models will shed light on how the host colonic environment impacts gene expression in these health-associated bacteria.
Altogether, REGUBIOME will provide proof of the importance of gut bacterial gene regulation networks to host health. Besides, by uncovering gene regulatory mechanisms in microbiome bacteria, REGUBIOME will provide novel actionable targets for microbiota modulation strategies.ver más
Seleccionando "Aceptar todas las cookies" acepta el uso de cookies para ayudarnos a brindarle una mejor experiencia de usuario y para analizar el uso del sitio web. Al hacer clic en "Ajustar tus preferencias" puede elegir qué cookies permitir. Solo las cookies esenciales son necesarias para el correcto funcionamiento de nuestro sitio web y no se pueden rechazar.
Cookie settings
Nuestro sitio web almacena cuatro tipos de cookies. En cualquier momento puede elegir qué cookies acepta y cuáles rechaza. Puede obtener más información sobre qué son las cookies y qué tipos de cookies almacenamos en nuestra Política de cookies.
Son necesarias por razones técnicas. Sin ellas, este sitio web podría no funcionar correctamente.
Son necesarias para una funcionalidad específica en el sitio web. Sin ellos, algunas características pueden estar deshabilitadas.
Nos permite analizar el uso del sitio web y mejorar la experiencia del visitante.
Nos permite personalizar su experiencia y enviarle contenido y ofertas relevantes, en este sitio web y en otros sitios web.