Towards the mechanism and function of tunneling nanotube TNT dependent interc...
Towards the mechanism and function of tunneling nanotube TNT dependent intercellular exchange of cargo
Tunneling nanotubes (TNTs), recently discovered thin membrane channels connecting distant cells, represent the underlying structure of a previously unrecognized type of cell-to-cell communication. To date, a growing number of cell...
ver más
¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video
Información proyecto FP7 TNT FUNCTION
Líder del proyecto
HOGSKULEN PA VESTLANDET
No se ha especificado una descripción o un objeto social para esta compañía.
Presupuesto del proyecto
205K€
Fecha límite de participación
Sin fecha límite de participación.
Descripción del proyecto
Tunneling nanotubes (TNTs), recently discovered thin membrane channels connecting distant cells, represent the underlying structure of a previously unrecognized type of cell-to-cell communication. To date, a growing number of cell types have been found to use TNTs for the intercellular exchange of diverse cargoes ranging from cytoplasmic signalling molecules such as calcium ions to small vesicles of endocytic origin. During the past year, pathogens such as the human immunodeficiency virus and prions were also found to spread TNT-dependently between cells. Given the emerging wide range of implications of TNTs in the field of biomedical research, it is important to first learn the basic principles and mechanisms of TNT-dependent cell-to-cell interactions. I therefore propose to focus on three major questions: First, I will characterize the type of endocytic vesicles transiting through TNTs using flow cytometry and quantitative live cell imaging to monitor the transfer of endosomal markers (Rab GTPases) through TNTs. Secondly, using the same methodological approach, I will analyze which myosin motor(s) is (are) involved in the active transport of vesicles through TNTs. Third, by employing a proteomic screen assay, I aim at characterizing the entire set of proteins/signalling molecules transferred through TNTs.