Hola,
¿eres nuevo aquí?

Regístrate gratis y conecta tu empresa con financiación pública, partners y proyectos.

Tengo cuenta

Regístrate

Ver video

Human-Ribogenesis

Financiado

Structural Biology of Human Ribosome Biogenesis

The emergence of numerous diseases caused by defective assembly of ribosomes calls for a deeper understanding of human ribosome biogenesis. Yet, in human cells the assembly of ribosomal subunits is a vital process of daunting comp... ver más

30/09/2025

LMU MUENCHEN

2M€

Presupuesto del proyecto: 2M€

Líder del proyecto

LUDWIGMAXIMILIANSUNIVERSITAET MUENCHEN No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Ver 2 Participantes

Financiación concedida El organismo H2020 notifico la concesión del proyecto el día 2020-09-18

ERC-2019-ADG: ERC Advanced Grant

I+D

Cerrada hace 5 años

0% 100%

2 Participantes

LMU MUENCHEN

2.08M€ | Lider

UPB ESPAÑA SA EN LIQUIDACION

Conecta tu I+D

¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video

Proyectos interesantes

BFU2010-15690 ESTUDIO DEL ENSAMBLAJE RIBOSOMICO EN SACCHAROMYCES CEREVISIA... 218K€ Cerrado

PID2020-117125RB-I00 IDENTIFICACION DE REGULADORES DE LA BIOGENESIS DEL RIBOSOMA... 217K€ Cerrado

PID2020-118378GB-I00 PROCESOS MOLECULARES IMPLICADOS EN LA FORMACION DE LOS RIBOS... 133K€ Cerrado

BES-2017-080876 ENSAMBLAJE DE SUBUNIDADES RIBOSOMICAS EUCARIOTICAS: ANALISIS... 93K€ Cerrado

BFU2017-88192-P ENSAMBLAJE Y MADURACION DE LA SUBUNIDAD PEQUEÑA DE LOS RIBOS... 97K€ Cerrado

T-FRAME Real time analysis of ribosomal frameshifting and its impact... 1M€ Cerrado

Duración del proyecto: 60 meses Fecha Inicio: 2020-09-18
Fecha Fin: 2025-09-30

Líder del proyecto

LUDWIGMAXIMILIANSUNIVERSITAET MUENCHEN No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 2M€

Descripción del proyecto The emergence of numerous diseases caused by defective assembly of ribosomes calls for a deeper understanding of human ribosome biogenesis. Yet, in human cells the assembly of ribosomal subunits is a vital process of daunting complexity, which requires several hundreds of assembly factors (AFs). Together with small nucleolar RNAs (snoRNAs) these factors facilitate a cascade of modification, processing and folding events of the ribosomal RNA (rRNA) that are tightly coordinated with the incorporation of a large set of ribosomal proteins between the nucleolus and the cytoplasm. This process has been extensively studied in fungal model organisms such as S. cerevisiae, however, assembly and rRNA processing pathways differ significantly between yeast and mammals. Many unique AFs have been identified in humans and several diseases, so-called ribosomopathies, have been assigned to ribosome biogenesis defects. Yet, so far only a single publication (Ameismeier et al., 2018) provides direct molecular information on the structural basis and architectural transitions of human ribosome maturation. Therefore, I propose to provide a near complete structural inventory of human ribosome biogenesis by using state-of-the-art cryo-electron microscopy (cryo-EM) on purified native pre-ribosomal particles. Corresponding analysis by chemical cross-link mass spectrometry, Nanopore sequencing and cryo-tomography as well as functional assays probing for nuclear export and RNA processing defects will gain complementary functional information. Finally, shot-gun cryo-EM of total pre-40S, pre-60S and 90S intermediates will be established in order to quantitatively characterize the equilibrium flow of ribosome assembly in normal and challenged human cells. Together, these insights will provide the basis for a mechanistic understanding of human ribosome biogenesis and will thereby lay the foundation for better relating this process to regulatory pathways and disease.

Conecta tu I+D

Entra hoy

¿Olvidé mi contraseña?

Financiación

Empresas

CTIs/Universidades

Proyectos

Investigadores