Hola,
¿eres nuevo aquí?

Regístrate gratis y conecta tu empresa con financiación pública, partners y proyectos.

Tengo cuenta

Regístrate

Ver video

DSBSunrise

Financiado

Cerrado

Identifying the steps required for meiotic DNA double strand break formation

At the onset of prophase of the first meiotic division, meiotic cells undergo complex molecular events with the induction of several hundred DNA double-strand breaks. These DNA breaks are required because they initiate recombinati... ver más

30/11/2026

CNRS

2M€

Presupuesto del proyecto: 2M€

Líder del proyecto

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Ver 2 Participantes

Financiación concedida El organismo H2020 notifico la concesión del proyecto el día 2020-05-12

ERC-2019-ADG: ERC Advanced Grant

I+D

Cerrada hace 5 años

0% 100% 100%

Información adicional privada

No hay información privada compartida para este proyecto. Habla con el coordinador.

2 Participantes

CNRS

2.17M€ | Lider

OLAZABAL Y HUARTE S A

612.60K€ | Participante

Conecta tu I+D

¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video

Proyectos interesantes

BrokenGenome Breaking and rebuilding the genome mechanistic rules for th... 1M€ Cerrado

DSB Architect The role of chromosome conformation in DNA double strand bre... 174K€ Cerrado

BFU2013-43965-P ESTUDIO DE LOS MECANISMOS QUE REGULAN LA PROGRESION DE LA PR... 121K€ Cerrado

HOTMEIOSIS Meiotic recombination How where and why? Mechanisms and Im... 2M€ Cerrado

BFU2012-35748 EL "CHECKPOINT" DE RECOMBINACION MEIOTICA: REGULACION EPIGEN... 126K€ Cerrado

CrossOver Meiotic crossing over from spatial distribution to in situ... 2M€ Cerrado

Duración del proyecto: 78 meses Fecha Inicio: 2020-05-12
Fecha Fin: 2026-11-30

Líder del proyecto

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 2M€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto At the onset of prophase of the first meiotic division, meiotic cells undergo complex molecular events with the induction of several hundred DNA double-strand breaks. These DNA breaks are required because they initiate recombination between homologous chromosomes and to allow chromosome segregation during meiosis. They are essential for fertility. However, they represent a major challenge for genome integrity. It is thought that meiotic DNA break formation is under tight control to ensure that all breaks are properly repaired to maintain genome integrity. But how this control implemented is unknown. We postulate that three critical steps take place to ensure meiotic DNA break formation at the right time, right place, and right frequency. We will test this hypothesis by addressing in mice the three following questions: Q1: We will ask whether a homology-sensing process brings homologous chromosomes in spatial proximity before DNA break formation to improve DSB repair efficiency and avoid topological conflicts. If this is the case, we will determine the molecular mechanism. Q2: We will determine whether the genomic sites undergoing DNA breakage interact with structural components of chromosome axes before break formation, and how. This interaction is predicted to be necessary for proper DSB repair. Q3: We will determine how DNA cleavage is activated. We will do this through in vitro reconstitution of meiotic DSB formation. Answering these key questions will be possible by using in vivo and in vitro approaches. We will pioneer in vitro meiotic differentiation of mouse embryonic stem cells to overcome the current limitations for identifying novel components and interactions. We will thus decipher how a molecular machinery that has evolved from a DNA type II topoisomerase family has been selected and modified to promote a complex reaction initiated by DNA cleavage at multiple sites along chromosomes followed by their repair by homologous recombination.

Conecta tu I+D

Entra hoy

¿Olvidé mi contraseña?

Financiación

Empresas

CTIs/Universidades

Proyectos

Investigadores