Dynamics and heterogeneity of early viral infection
Viruses are simple replicative units built from two components: a nucleic acid and a small number of associated proteins. Upon entering a cell, viruses co-opt the hosts machineries to copy their genetic material, while shutting do...
ver más
¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video
Proyectos interesantes
EUIN2015-62632
DINAMICAS DE POBLACIONES DE VIRUS DE RNA A ESCALA DE CELULAS...
10K€
Cerrado
PRE2018-083635
BASES ESTRUCTURALES DE LA INFECION VIRAL: MAQUINARIAS REPLIC...
93K€
Cerrado
PRE2021-098900
EL PAPEL DE LAS INTERACCIONES PROTEINA-PROTEINA Y PROTEINA-A...
101K€
Cerrado
RYC-2015-17517
Interaction of RNA Viruses with the Host Cell
309K€
Cerrado
BFU2014-58929-R
BASES ESTRUCTURALES Y MOLECULARES DE LA PROLIFERACION VIRAL....
230K€
Cerrado
BIO2017-83906-P
BASES ESTRUCTURALES DE LA INFECION VIRAL: MAQUINARIAS REPLIC...
315K€
Cerrado
Información proyecto VirIm
Duración del proyecto: 66 meses
Fecha Inicio: 2022-06-08
Fecha Fin: 2027-12-31
Descripción del proyecto
Viruses are simple replicative units built from two components: a nucleic acid and a small number of associated proteins. Upon entering a cell, viruses co-opt the hosts machineries to copy their genetic material, while shutting down the hosts antiviral immune response. The success of viral infection differs dramatically from cell to cell through poorly understood mechanisms, resulting in heterogenous propagation through tissues, and ultimately, heterogeneous disease progression.
Although viruses have been studied for decades, the earliest steps of cellular infection have remained hidden, because only a few viral molecules are present at this stage, which presents formidable challenges for molecular analyses. Yet these initial events are critical for achieving a successful infection; transcription, translation and replication must be perfectly balanced to rapidly scale virus production before antiviral signalling pathways are activated. To overcome this barrier, we recently developed a first-in-kind imaging technology for simple positive-sense single stranded RNA (+ssRNA) viruses that transforms our ability to visualize early viral infection processes.
In this proposal, we will expand our single–molecule toolbox to gain molecular insights into early viral infection of the more complex group of negative-sense RNA (-ssRNA) viruses. We will focus on the respiratory syncytial virus (RSV), a -ssRNA virus which can be deadly in infants and vulnerable adults, and lacks effective treatments. Specifically, we will determine i) how viral transcription and replication are coordinated on single viral genomic RNA molecules to optimize early viral propagation, ii) what causes the early viral infection heterogeneity, and iii) how heterogeneity in early viral infection impacts infection outcome. Using our novel approaches, we will gain a deep understanding of viral biology, which will eventually inform therapeutic interventions.