Tracing single-cell scale chemical signaling between interacting soil fungi
Multiple species of fungi co-exist in soils and play an important role in biogeochemical cycles. To survive in a resource limited environment, they have developed the means for interspecific communication and warfare via an arsena...
Multiple species of fungi co-exist in soils and play an important role in biogeochemical cycles. To survive in a resource limited environment, they have developed the means for interspecific communication and warfare via an arsenal of secreted secondary metabolites. Specific ecological role of those metabolites and the extent to which they affect biogeochemical cycling during fungal interactions remains unknown. Because they are secreted and act at a single-cell scale, tracing them ‘then and there’ can aid in identifying potential triggers for their production and clarifying their function. Currently used methods have either insufficient resolution or are destructive, and are not suitable for such analyses. Here, I will use my expertise in spectroscopy techniques to (1) establish experimental protocols for the single-cell scale fungal secondary metabolite identification and characterization using surface-enhanced Raman scattering (SERS) microspectroscopy - a method that employs optical properties of gold nanoparticles for molecule specific sensing and that has been shown in biomedical research to have an extraordinary potential for studying microbial metabolic processes. I will combine it with microfluidics based soil chips, that provide visual access to and mimic real ecosystems via control over biotic and abiotic environment of soil microbes. Ultimately, my aim is to offer the community of soil fungal ecologists with a game-changing new tool to study ecosystem functions of secondary metabolites in more realistic settings. I will then use the approach to (2) determine how interspecific fungal interactions under varying nutrient conditions affect the composition of their secondary metabolome and its functions live and at single-cell scale. Additional transcriptome analysis will (3) reveal fungal genes involved in up- or downregulation of the metabolite biosynthesis, but also extracellular enzyme production for organic matter degradation and nutrient acquisition.ver más
02-11-2024:
Generación Fotovolt...
Se ha cerrado la línea de ayuda pública: Subvenciones destinadas al fomento de la generación fotovoltaica en espacios antropizados en Canarias, 2024
01-11-2024:
ENESA
En las últimas 48 horas el Organismo ENESA ha otorgado 6 concesiones
01-11-2024:
FEGA
En las últimas 48 horas el Organismo FEGA ha otorgado 1667 concesiones
Seleccionando "Aceptar todas las cookies" acepta el uso de cookies para ayudarnos a brindarle una mejor experiencia de usuario y para analizar el uso del sitio web. Al hacer clic en "Ajustar tus preferencias" puede elegir qué cookies permitir. Solo las cookies esenciales son necesarias para el correcto funcionamiento de nuestro sitio web y no se pueden rechazar.
Cookie settings
Nuestro sitio web almacena cuatro tipos de cookies. En cualquier momento puede elegir qué cookies acepta y cuáles rechaza. Puede obtener más información sobre qué son las cookies y qué tipos de cookies almacenamos en nuestra Política de cookies.
Son necesarias por razones técnicas. Sin ellas, este sitio web podría no funcionar correctamente.
Son necesarias para una funcionalidad específica en el sitio web. Sin ellos, algunas características pueden estar deshabilitadas.
Nos permite analizar el uso del sitio web y mejorar la experiencia del visitante.
Nos permite personalizar su experiencia y enviarle contenido y ofertas relevantes, en este sitio web y en otros sitios web.