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LISGENOMICS

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Study of the Listeria monocytogenes gene expression profile in ready to eat food...

Study of the Listeria monocytogenes gene expression profile in ready to eat foods of animal origin by the application of the omics and the bioinformatics biostatistics disciplines Although the rate of food contamination with Listeria monocytogenes is low, the mortality rate that is caused by this microorganism is high, and for this reason, it is placed on the top of the list of pathogens of concern for the... ver más

01/05/2014

UNITO

50K€

Presupuesto del proyecto: 50K€

Líder del proyecto

UNIVERSITA DEGLI STUDI DI TORINO No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo FP7 notifico la concesión del proyecto el día 2014-05-01 No tenemos la información de la convocatoria

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1 Participantes

UNITO

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Líder del proyecto

UNIVERSITA DEGLI STUDI DI TORINO No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 50K€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto Although the rate of food contamination with Listeria monocytogenes is low, the mortality rate that is caused by this microorganism is high, and for this reason, it is placed on the top of the list of pathogens of concern for the public health and consequently for the food industry. L. monocytogenes has emerged as a food-borne pathogen the last decades, due to changes in food processing, consumer habits and the demand for minimally processed foods. The molecular mechanism of virulence has gained a lot of attention and many steps of this complex process have been elucidated to various levels. All L. monocytogenes strains found in foods should be considered to be pathogenic. However, the relative virulence of individual L. monocytogenes isolates can vary substantially. The genetic basis underlying these virulence differences is not yet understood. Differences in gene content exist between strains of different serovars and origins. Differences among strains could also be due to different gene expression/regulation of the core genes of the microorganism. The ability to rapidly determine the pathogenic potential of L. monocytogenes strains is integral to the control and prevention campaign against listeriosis. Throughout the years, different approaches have been employed to assess virulence: in vivo bioassays, in vitro cell assays and targeting virulence-associated proteins and genes. However, these methods have disadvantages such as the need for laboratory animals (bioassays) and the time constraints. Furthermore, targeting virulence genes gives only an indication of the virulence potential of strains. Nowadays, powerful alternatives such as microarrays and reverse transcriptional quantitative polymerase chain reaction (RT-qPCR) are available that can assist in the definition of the virulence potential of L. monocyotogenes strains by the means of modelling.

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