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PHAGE

Financiado

Structure and dynamics of PHage Attachment and Genome Ejection (PHAGE)

Phages have the potential to be used in therapy because they can infect and kill pathogenic bacteria, including those resistant to antibiotics. Although phages are simple, details of their life cycle remain unknown. To start the i... ver más

31/07/2025

150K€

Presupuesto del proyecto: 150K€

Líder del proyecto

Masarykova univerzita No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Financiación concedida El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-05-10

HORIZON-WIDERA-2022-TALENTS-04-01: Fostering balanced brain circulatio... ExpectedOutcome:This action will build on the MSCA Postdoctoral Fellowships 2022 action (HORIZON-MSC...

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Cerrada hace 2 años

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1 Participantes

150.44K€ | Lider

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Duración del proyecto: 26 meses Fecha Inicio: 2023-05-10
Fecha Fin: 2025-07-31

Líder del proyecto

Masarykova univerzita No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 150K€

Descripción del proyecto Phages have the potential to be used in therapy because they can infect and kill pathogenic bacteria, including those resistant to antibiotics. Although phages are simple, details of their life cycle remain unknown. To start the infection, phages bind to and eject their genome into bacteria. During adsorption, the phage baseplate recognises receptors in the bacterial cell wall, and therefore determines the phage specificity. Structural knowledge of baseplates will enable the rational engineering of phage host range. Before ejection, the phage genome is stored inside the capsid under high pressure. Although numerous studies of genome ejection have been performed, the understanding of this process is still incomplete because, until now, no suitable methods for studying in vivo ejection existed. The adsorption and genome ejection are closely related processes that can only be best understood together. In this project, hosted at Dr Plevka’s laboratory (CEITEC), I aim to determine the high-resolution structure of the phiKZ baseplate, one of the largest known bacteriophages, using cryogenic Transmission Electron Microscopy. The obtained structural information will be complemented by the characterisation of the genome ejection dynamics in vivo. For this purpose, I will use cryogenic Scanning Transmission Electron Microscopy to quantify the amount of DNA in phage heads at various stages of the infection. Furthermore, I will also use holotomography to visualise the ejection dynamics. There is still much to discover regarding bacterial adsorption and genome delivery. Thanks to my experience in phage and structural biology, I will integrate this project's results to shed light on the first steps of the phage life cycle, key to the infection of bacteria. My results will expand the understanding of phage infection and enable the retargeting of phages to specific bacterial strains, helping to develop phage therapy since antibiotic resistance is a rising health problem worldwide.

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