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PIGSNP

Financiado

Cerrado

SNPs of high utility within European commercial pig breeds

Within the PigSNP project SNPs will be identified that are known to be segregating within commercial European pig breeds. Using the Solexa sequencing technique a representation of the genome is directly sequenced to high redundanc... ver más

31/08/2010

170K€

Presupuesto del proyecto: 170K€

Líder del proyecto

WAGENINGEN UNIVERSITY No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo FP7 notifico la concesión del proyecto el día 2010-08-31 No tenemos la información de la convocatoria

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Información adicional privada

No hay información privada compartida para este proyecto. Habla con el coordinador.

1 Participantes

0.00€ | Lider

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Líder del proyecto

WAGENINGEN UNIVERSITY No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 170K€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto Within the PigSNP project SNPs will be identified that are known to be segregating within commercial European pig breeds. Using the Solexa sequencing technique a representation of the genome is directly sequenced to high redundancy. This is achieved by digesting the DNA with a restriction enzyme and sequencing fragments within a specific size range (i.e. 250 bp). The strategy used is based on sequencing a mixture of the DNA from 5 individuals of a single commercial breed to 20 X depth. Furthermore, the use of 5 individuals per breed (10 haplotypes) will ensure the identification of SNPs with high minor allele frequencies. This approach ensures that SNPs identified will be informative within the commercial line and therefore will be useful not only in experimental QTL crosses but also for genomic selection in current commercial breeding lines. The combination of new generation sequence technology (Solexa) combined with WITHIN breed SNP identification of major SNPs is particular innovative and timely. The SNPs identified will be available to be included in high density SNP panels (within existing international ongoing collaborations) that will be used to genotype available commercial pig breeds. This will aid in the further fine mapping of previously identified QTL within these commercial crosses. Within the project we will use the identified SNPs for fine mapping 2-3 previously identified QTL on chromosomes 2 and 4 identified within an experimental MeishanxEuropean white cross and within a commercial finisher population respectively. Both populations have been used extensively for the identification of several QTL for fatness, carcass and meat quality traits. Within the project it is aimed to identify between 10 and 20 thousand SNPs each for 4 different commercial breeds. QTL fine mapping will be done using the Illumina Golden Gate assay.

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