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HAHN_PFL_TETRONATES

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Identification of a general biosynthetic pathway of 3 acyltetronates and further...

Identification of a general biosynthetic pathway of 3 acyltetronates and further investigation of the biosynthetic pathway of the ionophoric polyethers tetronasin and tetronomycin A main aim of the research project is the elucidation of a general biosynthetic pathway of 3-acyltetronates. For this purpose, enzymes, which were identified in the gene clusters of the phosphatase inhibitor RK-682 and the quasi-e... ver más

31/07/2011

THE CHANCELLOR MAS...

171K€

Presupuesto del proyecto: 171K€

Líder del proyecto

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UN... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo FP7 notifico la concesión del proyecto el día 2011-07-31 No tenemos la información de la convocatoria

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1 Participantes

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Líder del proyecto

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UN... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 171K€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto A main aim of the research project is the elucidation of a general biosynthetic pathway of 3-acyltetronates. For this purpose, enzymes, which were identified in the gene clusters of the phosphatase inhibitor RK-682 and the quasi-enantiomeric ionophoric polyethers tetronasin and tetronomycin to be potentially responsible for tetronate formation will be heterologously expressed in E.coli. The purified enzymes will be applied for the development of in vitro assays with synthetically prepared surrogates. Besides the identification of a distinct biosynthetic pathway of 3-acyltetronates, the use of structurally diverse synthetic precursors will allow to draw conclusions about the substrate specifity of these enzymes. After expression of further enzymes from the tetronasin and the tetronomycin gene cluster, these enzymes will also be incubated with suggestive synthetic precursor analogues. Understanding the mechanism of 3-acyltetronate formation in RK-682 and the ionophoric polyethers tetronasin and tetronomycin will help to elucidate the mechanisms, which leads to the formation of other tetronate containing natural products like kijanimicin and abyssomycin. The information gained from these experiments will afford the application of the tetronate forming enzymes in the frame of combinatorial biosynthesis. It will permit the incorporation of tetronate forming enzymes into artificial biosynthetic assembly lines to generate novel and more potent agents by directed biosynthesis. Furthermore, all tailoring enzymes, e.g. these arranging for the highly stereoselective ring closures, will find an application in biocatalytic transformations once they were identified. Finally, elucidation of the whole post-PKS steps will help to understand the oddity of nature that created two complex, nearly perfect enantiomers, tetronasin and tetronomycin, which have more or less the same function and mode of action.

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