Hola,
¿eres nuevo aquí?

Regístrate gratis y conecta tu empresa con financiación pública, partners y proyectos.

Tengo cuenta

Regístrate

Ver video

DESB

Financiado

Cerrado

Directed Evolution in vivo enabled through genetic circuits in a Synthetic Biolo...

Directed Evolution in vivo enabled through genetic circuits in a Synthetic Biology approach Given our limited ability to rationally design proteins to our needs, their supply for biotechnological solutions leading to a sustainable Bioeconomy is relying on proteins found in nature and methods for deriving improved version... ver más

31/05/2016

IMPERIAL COLLEGE O...

222K€

Presupuesto del proyecto: 222K€

Líder del proyecto

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND ME... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo FP7 notifico la concesión del proyecto el día 2016-05-31 No tenemos la información de la convocatoria

0% 100% 100%

Información adicional privada

No hay información privada compartida para este proyecto. Habla con el coordinador.

1 Participantes

IMPERIAL COLLEGE OF SCIENCE...

0.00€ | Lider

Conecta tu I+D

¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video

Proyectos interesantes

BIO2014-54411-C2-2-R DISEÑO MATEMATICO DE ESTRATEGIAS PARA LA INGENIERIA METABOLI... 85K€ Cerrado

DPI2017-82896-C2-2-R DISEÑO, CARACTERIZACION Y AJUSTE OPTIMO DE BIOCIRCUITOS SINT... 145K€ Cerrado

DPI2017-82896-C2-1-R DISEÑO, CARACTERIZACION Y AJUSTE OPTIMO DE BIOCIRCUITOS SINT... 154K€ Cerrado

BIONICbacteria Integrating a novel layer of synthetic biology tools in Pseu... 2M€ Cerrado

SynthStripe Synthetic gene regulatory networks for single stripe gene ex... 169K€ Cerrado

PROTLEGO Development of an accessible platform for ex vivo site spec... 1M€ Cerrado

Líder del proyecto

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND ME... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 222K€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto Given our limited ability to rationally design proteins to our needs, their supply for biotechnological solutions leading to a sustainable Bioeconomy is relying on proteins found in nature and methods for deriving improved versions. Proteins evolved their structure to fulfil certain functions in very specific niches, but these do not resemble optimal properties in the new context of biotechnological applications. Hence, improving the activity, affinity and stability of a protein is key, but technologically challenging. The current methods for improving proteins through directed evolution require intense human intervention and are limited in their ability to sample sequence space beyond single and double mutations. This project will establish a novel system, in which the whole process of mutation and selection takes place in vivo inside an E. coli host, and can be controlled through a genetic network designed using a Synthetic Biology approach. It will allow for the exploration of a larger sequence space during the evolutionary process, while controlling mutagenesis rate and selection stringency. Our preliminary data demonstrates a groundbreaking novel method to target mutagenesis in vivo. I will extend its mutation diversity by recruiting engineered error prone polymerases to the site of the targeted DNA damage. Using a SynBio approach, I will develop a control circuitry, which will stop the targeted mutagenesis activity, once a variant fulfils the selection criteria. While protecting the identity of the selected sequence, it will mark the successful cell by expression of a fluorescent reporter for selection. In a prototype application I will generate variants of the Quorum Sensing transcription factor, LuxR, with high affinities to different signalling molecules – a potential toolset for orthogonal cellular signalling applications in industrially relevant co-cultures. The resulting directed evolution system will be readily adaptable to different proteins.

Conecta tu I+D

Entra hoy

¿Olvidé mi contraseña?

Financiación

Empresas

CTIs/Universidades

Proyectos

Investigadores