circlePLANT
Financiado
Deciphering the eccDNA inheritance with novel plant eccDNA genetic tools
Enhancing plants’ natural innate and immune systems for the generation of primed plants that will better withstand stressors, can be an effective way to reduce losses in crop production. These systems are broadly defined under the...
Enhancing plants’ natural innate and immune systems for the generation of primed plants that will better withstand stressors, can be an effective way to reduce losses in crop production. These systems are broadly defined under the plant transgenerational inheritance (TI) of stress memory, which is based on the heritability of attained traits caused by environmental conditions. I propose a new player in plant TI: the extrachromosomal circular DNA (eccDNA). EccDNA is recognized as a major source of genetic variation behind key adaptive traits. I hypothesize that stress-responsive eccDNAs in the soma of a plant can be considered as adaptive toolkits, that can be transferred to the gametes during meiosis, and thus to the progeny. Thus, I have developed an ambitious but feasible plan, to bridge two major gaps in this context: (1) We require innovative technologies to prove causation, so I will develop the first and novel genetic toolbox to study eccDNAs in plants, by targeted circularization driven by the novel CRISPR-C system; (2) We lack knowledge about how eccDNAs are inherited, so I will characterize the transmission of eccDNAs from the soma to the gametophyte in Arabidopsis, through genome-scale (Circle-seq) and cytological analysis (FISH). CirclePLANT offers a unique opportunity for the development of a new line of research, with potentially remarkable impact because it will explore the bases for eccDNA-based technologies that may be useful in crop breeding, ultimately leading to the generation of stable climate-smart crops, which fits with the environmental objectives of the European Common Agricultural Policy (CAP) of improving crop yields by applying low-input agricultural practices.
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Duración del proyecto: 26 meses
Fecha Inicio: 2022-06-03
Fecha Fin: 2024-08-31
Fecha Fin: 2024-08-31
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2024-08-31
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2021-PF-01-01:
MSCA Postdoctoral Fellowships 2021
Cerrada
hace 3 años
Presupuesto
El presupuesto total del proyecto asciende a
215K€
Líder del proyecto
KOBENHAVNS UNIVERSITET
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5