DeDNAed
Financiado
Cluster decorated recognition elements on DNA origami for enhanced raman spectroscopic detection methods
The project DeDNAed is intended to develop a novel, innovative biosensing platform whose advantages and benefits are in terms of sensitivity, versatility and being ultrafast by an optical approach. Our platform will be based on th...
The project DeDNAed is intended to develop a novel, innovative biosensing platform whose advantages and benefits are in terms of sensitivity, versatility and being ultrafast by an optical approach. Our platform will be based on the assembly and integration of sensing elements (transducer and bioreceptor) by DNA origami. The DNA origami will serve as a nano bread board in order to precisely control the position of these elements and thus the sensor architecture at the nanometer scale. Metallic atomic clusters (ACs) are integrated into a biological marker molecule (DNA or antibody) and thus represent the biological sensor element. This is specifically integrated into a nanoarray made of additional metallic nanoparticles precisely controlled by a DNA origami template and will lead to a significant increase in signal. DNA origami serves as an individually inter- and intramolecularly programmable nano bread board. A DNA origami consists of a single strand of DNA, folded by a thermal treatment and certain staple strands into any shapes (2D as well as 3D, dimensions between 10 and several 100 nanometers). So-called sticky ends on the surface of the DNA origami offer the possibility of an individual implementation of the sensing elements and NPs, by means of correspondingly complementary oligonucleotides with a resolutions of 2 nm. When the analyte is connected to the sensor element, a change in the Raman signal can thus be detected without major delay using surface-enhanced Raman spectroscopy (SERS). This sensor method is not bound to a specific biomarker molecule for the sensor element, but can be transferred to different marker molecules. This means a high degree of flexibility in the area of application, from medical technology to food monitoring. In addition, a transfer of the DNA origami-based sensor platform to flexible, textile substrates is carried out using lipid bi layers and the Langmuir-Blodgett method for later use as a wipe test or medical wearable.
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Duración del proyecto: 35 meses
Fecha Inicio: 2021-03-01
Fecha Fin: 2024-02-29
Fecha Fin: 2024-02-29
Línea de financiación: concedida
El organismo H2020 notifico la concesión del proyecto el día 2024-02-29
H2020
No se conoce la línea exacta de financiación, pero conocemos el organismo encargado de la revisión del proyecto.
Presupuesto
El presupuesto total del proyecto asciende a
3M€
Líder del proyecto
TECHNISCHE UNIVERSITAET CHEMNITZ
No se ha especificado una descripción o un objeto social para esta compañía.
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