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CHAMELEO

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Cellular Hypoxia Alters DNA Methylation through Loss of Epigenome Oxidation

DNA methylation is integral to the regulation of gene transcription but is deregulated in cancer. Mechanisms causing this dysregulation are however poorly understood. Although DNA methylation mechanisms were described for several... ver más

30/04/2016

VLAAMS INSTITUUT...

75K€

Presupuesto del proyecto: 75K€

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TRL 4-5

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Financiación concedida El organismo FP7 notifico la concesión del proyecto el día 2016-04-30 No tenemos la información de la convocatoria

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VIB VZW No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 75K€

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Descripción del proyecto DNA methylation is integral to the regulation of gene transcription but is deregulated in cancer. Mechanisms causing this dysregulation are however poorly understood. Although DNA methylation mechanisms were described for several decades ago, the enzymes responsible for DNA demethylation were only discovered a few years ago: TET enzymes catalyze the conversion of 5-methylcytosine to 5-hydroxymethylcytosine (5hmC) through a reaction requiring oxygen and 2-oxoglutarate (2OG). Interestingly, decreased oxygen concentrations (hypoxia) are common in cancer, as is an altered 2OG metabolism. Hypoxia induces the activity of Hypoxia Inducible Factor (HIF) transcription factors, which alter gene expression in cells to counter and cope with hypoxia. It is however poorly characterized how DNA methylation influences HIF binding, and how HIF in turn interfaces with the epigenome. In CHAMELEO, we aim to investigate the influence of hypoxia on the epigenome and the resulting phenotypic response in cancer. Firstly, we hypothesize that promoters of tumor suppressor gene can be hypermethylated through inhibition of DNA demethylation at low oxygen concentrations, thus contributing to oncogenic transformation. To investigate this, we will study the dependence of TET DNA hydroxylases on oxygen and 2OG, as well as the overall and locus-specific changes upon hypoxia of their product (5hmC). Secondly, because much of the hypoxic response is executed through HIFs, we will investigate how HIF binding is influenced by DNA methylation and how HIFs interface with epigenetic modulators such as TETs. Experiments in vitro and in vivo will be combined with state-of-the-art high-throughput sequencing techniques, and are expected to provide an unambiguous and comprehensive view on the role of DNA methylation dynamics in hypoxia and cancer.

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