Descripción del proyecto
THE MAIN GOAL OF THIS COORDINATED PROJECT WILL FOCUS IN THE NITRATION PROCESS MEDIATED BY RNS WITH SPECIAL EMPHASIS IN THE BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF THE ENDOGENOUS CELLULAR TARGETS (PROTEINS, LIPIDS AND NUCLEIC ACIDS) AND ITS INVOLVEMENT IN SIGNALLING MECHANISM DURING THE PHYSIOLOGICAL PROCESSES OF GERMINATION AND PLANT GROWTH, AND IN ENVIRONMENT ABIOTIC STRESSES SUCH AS SALINITY, MECHANICAL WOUNDING, LOW AND HIGH TEMPERATURE IN HIGHER PLANTS, NITRIC OXIDE (NO) AND NO-DERIVED REACTIVE NITROGEN SPECIES (RNS) CAN MEDIATE SPECIFIC MODIFICATIONS IN BIOLOGICAL MACROMOLECULES THAT AFFECT THEIR FUNCTIONS, SO THEY COULD BE USED AS TOOLS TO REGULATE CELL METABOLISM AND AS INDICATORS OF PHYSIOLOGICAL DYSFUNCTION UNDER STRESS CONDITIONS, SPECIAL ATTENTION WILL PUT IN THE IDENTIFICATION AND CHARACTERIZATION OF LIPID AND PROTEIN NITRATION INCLUDING TRANSCRIPTION FACTORS (TFS) MODULATED BY RNS AS ADDITIONAL PATHWAYS OF GENE REGULATION BY NO, FURTHERMORE, IT WILL EXPLORE THE INVOLVEMENT OF NEW MOLECULES DESIGNATED AS REACTIVE SULPHUR SPECIES (RSS) IN THESE PROCESSES, THE MOLECULAR TARGETS OF NITRATION WILL BE IDENTIFIED AND CHARACTERIZED BY A COMBINATION OF COMPLEMENTARY APPROACHES SUCH AS PROTEOMIC (2 D-PAGE), HPLC, MASS SPECTROMETRY TECHNIQUES (TANDEM TIME-OF-FLIGHT MASS SPECTROMETER AND ELECTROSPRAY-IONIZATION TRIPLE QUADRUPOLE MS -ESI MS/MS-), SPECTROFLUOROMETRIC ANALYSIS, OZONE CHEMILUMINISCENCE AND ELECTRON PARAMAGNETIC RESONANCE (EPR) SPECTROSCOPY, THE CELLULAR LOCALIZATION OF THESE MOLECULES IN DIFFERENT TISSUES (ROOT, STEM AND LEAF) DURING SEED GERMINATION, GROWTH, AND UNDER ENVIRONMENTAL STRESS CONDITIONS WILL BE ALSO STUDIED BY CONFOCAL LASER SCANNING MICROSCOPY (CLSM) ANALYSIS USING FLUORESCENT PROBES AND SPECIFIC ANTIBODIES, THIS STUDY WILL BE CARRY OUT IN DIFFERENT PLANT SPECIES TO GET A MORE COMPREHENSIBLE ANALYSES SUCH AS PEA (PISUM SATIVUM L) AND ARABIDOPSIS (ARABIDOPSIS THALIANA L,) AS PLANT MODELS, AND OLIVE (OLEA EUROPAEA L), SUNFLOWER (HELIANTHUS ANNUS L) AND PEPPER (CAPSICUM ANNUUM L,) AS PLANTS OF AGRONOMICAL AND HORTICULTURAL INTEREST, ALL THESE MECHANISMS ARE AT PRESENT UNKNOWN IN PLANTS, AND THE OBTAINED RESULTS COULD HAVE BIOTECHNOLOGICAL APPLICATIONS IN ORDER REGULATE SEED GERMINATION AND PLANT DEVELOPMENT AS WELL TO INCREASE OUR UNDERSTANDING OF THE MECHANISMS THAT ENABLE PLANTS TO WITHSTAND ENVIRONMENTAL ABIOTIC STRESSES WHICH ARE CURRENTLY RESPONSIBLE FOR IMPORTANT DECREASES IN AGRICULTURAL OUTPUTS, IN THIS SENSE, SYNGENTA SEED, SA AND NUTESCA SL, OUR TWO COMPANIES INVOLVED IN PLANT PRODUCTIVITY AND PROTECTION THAT SUPPORT OUR COORDINATED PROJECT, OXIDO NITRICO\ESPECIES DE NITROGENO REACTIVO\GERMINACION\DESARROLLO\ESTRES NITROSATIVO\NITRACION