Innovating Works

CARBOCENTRE

Financiado
Activity Based Profiling of Glycoprocessing Enzymes for Human Health and a Susta...
Activity Based Profiling of Glycoprocessing Enzymes for Human Health and a Sustainable Society Enzymes that produce and degrade oligosaccharides and glycoconjugates are present in all kingdoms of life. The ability to visualize, modulate and understand these carbohydrate-active enzymes (CAZymes) therefore offers great potent... Enzymes that produce and degrade oligosaccharides and glycoconjugates are present in all kingdoms of life. The ability to visualize, modulate and understand these carbohydrate-active enzymes (CAZymes) therefore offers great potential for human health and sustainable industries. To provide a disruptive shift in our understanding, we adopt in this proposal a multidisciplinary approach combining structural biology, enzymology, computational chemistry, organic synthesis, and chemical biology, with major leaders in these fields part of our CARBOCENTRE Synergy Team. Three fundamental strands will specifically target and ‘capture’ glycoprocessing enzyme active sites. Biochemical and 3-D structural analyses will inform computational dissection of the reaction coordinate of key enzymes for human health and biotechnology processes. Building on our founding work on retaining glycosidases we will also target inverting glycosidases and glycosyltransferases. Following fundamental analyses, our probes will feed research in two major application domains of human health and biotechnology: 1. To provide visualization, diagnosis, and inhibitor assays and clinical lead compounds for enzymes in cancers and genetic diseases (lysosomal storage disorders). 2. To explore the natural diversity of CAZymes and to discover, quantify and optimize new enzymes for food and household applications and for biomass conversion to biofuels. In an iterative cycle, structural biology and enzymology (Davies, York), will inform, through structures of enzymes and enzyme-inhibitor complexes, theoretical and computational chemistry (Rovira, Barcelona), which in turn will guide the design and synthesis (Overkleeft, Leiden), of inhibitors and activity-based probes for ensuing chemical biology studies in the domains of biomedicine and biotechnology. ver más
31/05/2027
ULEI
9M€
Perfil tecnológico estimado
Duración del proyecto: 77 meses Fecha Inicio: 2020-12-18
Fecha Fin: 2027-05-31

Línea de financiación: concedida

El organismo H2020 notifico la concesión del proyecto el día 2020-12-18
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
ERC-2020-SyG: ERC Synergy Grant
Cerrada hace 5 años
Presupuesto El presupuesto total del proyecto asciende a 9M€
Líder del proyecto
UNIVERSITEIT LEIDEN No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5