Innovating Works

SymFlux

Financiado
The role of symbiont diversity in resilience to temperature fluctuations.
Beneficial microbes are essential for many animals and mutualistic interactions must be able to withstand environmental changes such as temperature fluctuations. However, with changing ocean temperatures induced by climate change,... Beneficial microbes are essential for many animals and mutualistic interactions must be able to withstand environmental changes such as temperature fluctuations. However, with changing ocean temperatures induced by climate change, it is unclear whether symbioses will be able to adapt, leaving marine organisms at risk. Therefore, this proposal will characterize how bacterial symbionts respond to changing temperatures and identify whether microbes can increase symbiotic resiliency to temperature stress. To pursue this topic, the symbiosis between the squid Sepiola affinis and its symbionts (V. fischeri and V. logei) will be used. The project uses a unique squid husbandry facility to pursue three main objectives. The first objective will characterize initial and persistent colonization in the S. affinis light organ. Using confocal microscopy and genetic labelling, strain behaviors during colonization will be described. The second objective will examine how temperature affects colonization. The third objective will screen Vibrio spp. for growth under temperature fluctuations. RNA sequencing and metabolomics will be used to identify molecular mechanisms underlying resilience to temperature fluctuations. Finally, genetic modification and both in-vitro and in-vivo growth assays will test whether molecular candidates promote temperature resilience. The project will have a two-way transfer of knowledge, with the researcher providing knowledge of Vibrio spp. and bacterial genetics with the host institution providing bioinformatics and small molecule analysis. The SymFlux project will position S. affinis as a model for symbiosis research in Europe as well as contribute knowledge of how symbioses can adapt to climate change. ver más
31/08/2026
SORBONNE UNIVERSIT...
212K€
Perfil tecnológico estimado
Duración del proyecto: 28 meses Fecha Inicio: 2024-04-17
Fecha Fin: 2026-08-31

Línea de financiación: concedida

El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2024-04-17
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2023-PF-01-01: MSCA Postdoctoral Fellowships 2023
Cerrada hace 1 año
Presupuesto El presupuesto total del proyecto asciende a 212K€
Líder del proyecto
SORBONNE UNIVERSITE No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5