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BIONANODIAMOND

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The development of a diamond based nanopore sensor for the detection and identif...

The development of a diamond based nanopore sensor for the detection and identification of DNA The detection of specific sequences of DNA is pivotal in the diagnosis of a number of infectious diseases, such as chlamydia. It is also important in detecting and identifying potential bio-warfare agents. Current methods of DNA d... ver más

05/04/2017

UNIVERSITY OF WARW...

282K€

Presupuesto del proyecto: 282K€

Líder del proyecto

UNIVERSITY OF WARWICK No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo FP7 notifico la concesión del proyecto el día 2017-04-05 No tenemos la información de la convocatoria

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1 Participantes

UNIVERSITY OF WARWICK

0.00€ | Lider

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Líder del proyecto

UNIVERSITY OF WARWICK No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 282K€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto The detection of specific sequences of DNA is pivotal in the diagnosis of a number of infectious diseases, such as chlamydia. It is also important in detecting and identifying potential bio-warfare agents. Current methods of DNA detection typically involve laboratory based sample analysis, which is slow and costly. This proposal seeks to address these drawbacks by developing an alternative approach based on nanopore unzipping. In this method the current-time transient is recorded for a single molecule of dsDNA as it denatures and passes through a protein pore. The current as the DNA blocks the pore, as well as the time taken for the dsDNA to 'unzip' (denature) is characteristic of the DNA sequence, length and the presence of any modifications. The proiten pore itself must be suspended in a lipid bilayer across a nm-diameter orifice that acts as a platform. Whilst identification of DNA targets with nanopore unzipping is expected to be quick and efficient, the platforms typically used for such measurements (mainly silicates) are fragile and lack bio-compatibility. This proposal seeks to bring the power of nanopore unzipping outside of the laboratory through the development of a robust, ultra-stable, platform constructed from diamond. The research conducted during this proposal could lead to a commercially viable device capable of detecting and identifying DNA at the point need, for example in medical diagnostics in a hospital or identifying potential bio-warfare agents in the field of operation.

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