Hola,
¿eres nuevo aquí?

Regístrate gratis y conecta tu empresa con financiación pública, partners y proyectos.

Tengo cuenta

Regístrate

Ver video

Ti-EM

Financiado

Cerrado

Methodological developments for time resolved single particle cryo EM

Protein synthesis and degradation, energy metabolism, signalling, processing of information-encoding polymers (RNA and DNA) are facilitated and regulated by molecular machines, protein complexes that undergo significant conformati... ver más

30/11/2022

VLAAMS INSTITUUT...

2M€

Presupuesto del proyecto: 2M€

Líder del proyecto

VIB VZW No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Ver 2 Participantes

Financiación concedida El organismo H2020 notifico la concesión del proyecto el día 2022-11-30

ERC-2016-COG: ERC Consolidator Grant

I+D

Cerrada hace 9 años

0% 100% 100%

Información adicional privada

No hay información privada compartida para este proyecto. Habla con el coordinador.

2 Participantes

VLAAMS INSTITUUT BIOTECHNOL...

1.78M€ | Lider

DERAZA IBERICO

Proyectos interesantes

EUKARYOTIC RIBOSOME

Medical Technol...

Applied Physics

2M€ Cerrado

BFU2008-01344

Medical Technol...

Applied Physics

417K€ Cerrado

CRYOTRANSLATION

Medical Technol...

Applied Physics

3M€ Cerrado

BFU2011-29038-C02-01

Medical Technol...

Applied Physics

455K€ Cerrado

3D-Xist

Medical Technol...

Applied Physics

183K€ Cerrado

NUCLEAR PORE COMPLEX

Medical Technol...

Applied Physics

170K€ Cerrado

Conecta tu I+D

¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video

Duración del proyecto: 69 meses Fecha Inicio: 2017-02-27
Fecha Fin: 2022-11-30

Líder del proyecto

VIB VZW No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 2M€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto Protein synthesis and degradation, energy metabolism, signalling, processing of information-encoding polymers (RNA and DNA) are facilitated and regulated by molecular machines, protein complexes that undergo significant conformational changes in time as they facilitate their function. To be able to control the biological processes rationally and with high precision it is essential to understand their mechanism at atomic level. A powerful way to gain detailed insight onto function of these proteins is to see these molecules at atomic resolution while they function. The high-resolution structures of biological macromolecules obtained by X-ray crystallography, NMR and more recently single particle electron cryogenic microscopy (cryo-EM) have provided insights onto the way many molecular machines are constructed. These methods generally provide snapshots of discrete long-lived states, visualizing the conformational changes along the reaction trajectory at high-resolution often remains an elusive objective. The aim of my proposal is to develop methods for visualizing transient conformations of protein complexes by time-resolved single particle cryo-EM. Time-resolved cryo-EM combines structural study with kinetics by freeze-trapping kinetic intermediates in a biological reaction and has potential to provide atomic-resolution ‘movies’ of functioning biological complexes. Technical limitations however so far restricted widespread use and utilization of the complete potential of this technique. Main part of this project is dedicated to development of microfluidic instruments for cryo-EM sample preparation. If successful, our approaches will allow trapping kinetic intermediates of molecular machines with millisecond time-resolution using picogram amounts of protein sample. The developed method will be applied to resolve key functional conformations of respiratory complex I and observe regulatory trajectories of ligand-gated ion channels.

Conecta tu I+D

Entra hoy

¿Olvidé mi contraseña?

Financiación

Empresas

CTIs/Universidades

Proyectos

Investigadores