PoreMADNeSS
Financiado
Massive parallel de novo design of sensing nanopores
Proteins embedded in membranes play key roles in maintaining cell integrity, homeostasis and communication. Emerging technologies (nanopore sequencing, synthetic cells, …) imitate biological systems and repurpose membrane protein...
Proteins embedded in membranes play key roles in maintaining cell integrity, homeostasis and communication. Emerging technologies (nanopore sequencing, synthetic cells, …) imitate biological systems and repurpose membrane protein for the transport and sensing of new analytes through synthetic membranes. These applications have fuelled the demand for (synthetic) membrane proteins with properties and functions not observed in nature. Structure-based computational protein design is revolutionizing many aspects of biotechnology but has almost exclusively focused on protein folding in water. The aim of PoreMADNeSS is to develop innovative strategies to enable the design of transmembrane β-barrels (TMBs), a class of membrane proteins with excellent properties to act as nanopore sensors. Using multidisciplinary approaches, we will address basic biophysical knowledge gaps that currently limit TMB design. The design of TMB folding in synthetic membranes gives access to a wealth of TMB sequences and structures not sampled by nature because of constraints associated with biogenesis and with the composition of biomembranes. We propose a combination of massive parallel de novo design and adaptive machine learning to explore this unknown TMB space, to gain crucial insight into the determinants of TMB folding and to develop robust design methods. As a proof-of-concept, PoreMADNeSS will focus on the design of steroid sensing nanopores. Our strategy is to design a cortisol binding site across the transmembrane channel, which would act as the reading head for single molecule fingerprinting. My lab was first to demonstrate the feasibility of TMB design and has established a design pipeline from computation to electrophysiology and biochemical characterization. This project has all the components to translate into transformative advances in nanopore sensing and sequencing by providing the nanopore R&D community with accurate and innovational computational design methodologies.
ver más
Duración del proyecto: 65 meses
Fecha Inicio: 2023-10-18
Fecha Fin: 2029-03-31
Fecha Fin: 2029-03-31
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-10-18
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
ERC-2023-STG:
ERC STARTING GRANTS
Cerrada
hace 2 años
Presupuesto
El presupuesto total del proyecto asciende a
2M€
Líder del proyecto
VIB VZW
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5