cryoSPARTAN
Financiado
in situ Structure Determination of Actin-binding Proteins Through a Novel Cryo-e...
in situ Structure Determination of Actin-binding Proteins Through a Novel Cryo-electron Microscopy Workflow
Cell motility is driven by a complex network of force-generating biological machinery. The key component in this machinery is the dynamic network of filamentous actin (F-actin) and actin binding proteins (ABPs) which maintain and...
Cell motility is driven by a complex network of force-generating biological machinery. The key component in this machinery is the dynamic network of filamentous actin (F-actin) and actin binding proteins (ABPs) which maintain and regulate the network. However, structural information for many ABP-actin complexes, as well as their in situ spatial distribution remain elusive. This is because ABPs cannot be easily studied in isolation, often exhibiting structural stability only when embedded in a complex filamentous network found within cells. This has hindered a complete understanding of actin network regulation in cell migration. Addressing this important question requires understanding exactly how ABPs select F-actin, and conversely how F-actin geometry recruits specific ABPs. Cryo-electron tomography (cryo-ET) can reveal both cellular ultrastructure and molecular details, but often is lower resolution than a single particle cryo-EM approach. Thus, innovative methods remain key to drive advancement in understanding in situ structures. In this fellowship I will combine my expertise of single particle cryo-electron microscopy with expertise of cryo-ET in the Schur lab to develop a novel hybrid single particle cryo-ET approach in order to reveal high-resolution structures and contextual information of ABPs bound to F-actin directly within cellular protrusions. ISTA is the ideal research institute due to abundant access to high-end electron microscopes necessary for methods development. The outcome of this action are tools for high resolution in situ structure determination and a better understanding of cell migration, a process deeply rooted in malignant metastasis. Results will be disseminated through key research conferences and high-impact open-access publications. Communication activities will be achieved through 3D rendered visual scientific illustrations targeting social media platforms and institute-organized public outreach events.
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Duración del proyecto: 24 meses
Fecha Inicio: 2023-03-09
Fecha Fin: 2025-03-31
Fecha Fin: 2025-03-31
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-03-09
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2022-PF-01-01:
MSCA Postdoctoral Fellowships 2022
Cerrada
hace 2 años
Presupuesto
El presupuesto total del proyecto asciende a
199K€
Líder del proyecto
INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5