T2D-EOMICS
Financiado
High throughtput electrophysiological measurements coupled with transcriptomics...
High throughtput electrophysiological measurements coupled with transcriptomics to reveal cellular dysfunction in type 2 diabetes
In this project, I will develop methods to simultaneously measure transcriptomes with single-cell resolution and perform high-throughput functional measurements of individual pancreatic islet cells. I will focus on β- and α-cells...
In this project, I will develop methods to simultaneously measure transcriptomes with single-cell resolution and perform high-throughput functional measurements of individual pancreatic islet cells. I will focus on β- and α-cells which regulate glucose levels by secreting the two main glucoregulatory hormones -insulin and glucagon- and whose dysfunction is associated with diabetes. The islet is a highly heterogenous mini-organ, making it an ideal tissue to study the relationship between molecular and functional heterogeneity. It has been recently developed the use of patch-clamp electrophysiology and single-cell RNA sequencing (patch-seq) using whole-cell electrophysiology. In this work, I will combine high-density microelectrode array technology (HD-MEA) and transcriptomic methods to identify changes that lead to dysfunctional cellular states in type 2 diabetes. To do so, I will build a cell cherry-picking system to perform single-cell RNA sequencing after electrophysiological measurements in the same islet cell. Then I will use this system to investigate subpopulations of α- and β-cells that have been previously identified in human islets from donors with type 2 diabetes using transcriptomic methods, but whose implications in cell and tissue function are yet unclear.
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Duración del proyecto: 24 meses
Fecha Inicio: 2023-03-08
Fecha Fin: 2025-03-31
Fecha Fin: 2025-03-31
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-03-08
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2022-PF-01-01:
MSCA Postdoctoral Fellowships 2022
Cerrada
hace 2 años
Presupuesto
El presupuesto total del proyecto asciende a
207K€
Líder del proyecto
GOETEBORGS UNIVERSITET
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5
N.D. |
Participante