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BIO2010-15384

Financiado

FABRICANDO TOMATES SALUDABLES: BIOPIEZAS PARA INTRAGENESIS Y MOLECULAR FARMING E...

FABRICANDO TOMATES SALUDABLES: BIOPIEZAS PARA INTRAGENESIS Y MOLECULAR FARMING EN SOLANACEAS THE POST-GENOMICS ERA HAS PUT AT THE DISPOSAL OF MODERN PLANT BREEDERS AN ENDLESS LIST OF GENETIC BUILDING BLOCKS FOR THE DESIGN OF NEW BIOTECHNOLOGICAL CROPS, AFTER A FIRST WAVE OF SINGLE-GENE TRANSGENICS WITH CONTROVERSIAL PUBLI... ver más

01/01/2010

CSIC

242K€

Presupuesto del proyecto: 242K€

Líder del proyecto

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGA... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 2-3 | 552M€

Financiación concedida El organismo AGENCIA ESTATAL DE INVESTIGACIÓN notifico la concesión del proyecto el día 2010-01-01 No tenemos la información de la convocatoria

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CSIC

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Líder del proyecto

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGA... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 2-3 | 552M€

Presupuesto del proyecto 242K€

Descripción del proyecto THE POST-GENOMICS ERA HAS PUT AT THE DISPOSAL OF MODERN PLANT BREEDERS AN ENDLESS LIST OF GENETIC BUILDING BLOCKS FOR THE DESIGN OF NEW BIOTECHNOLOGICAL CROPS, AFTER A FIRST WAVE OF SINGLE-GENE TRANSGENICS WITH CONTROVERSIAL PUBLIC ACCEPTANCE, GENOMIC INFORMATION AND TECHNOLOGY IS PAVING THE WAY FOR INCREASINGLY COMPLEX DESIGNS BASED IN MULTIPLE GENE ENGINEERING, THOSE DESIGNS AIMING AT THE PRODUCTION OF INEXPENSIVE HEALTH-PROMOTING COMPOUNDS ARE MOST LIKELY TO BE WELCOMED BY CONSUMERS, IN THIS PROJECT WE PLAN TO DEVELOP NEW MULTIGENE ASSEMBLING TOOLS AND USE THEM TO DEVELOP NOVEL TOMATO VARIETIES RICH IN ORALLY-ACTIVE HEALTH-PROMOTING COMPOUNDS, THE FIRST PROPOSED PRODUCT IS A POLYPHENOL-RICH TOMATO ENGINEERED BY INTRAGENESIS, I,E, USING EXCLUSIVELY TOMATO¿S OWN GENOME SEQUENCES, FLAVONOIDS AND ANTHOCYANINS ARE ANTI-OXIDANT POLYPHENOL COMPOUNDS PROVEN TO HAVE STRONG ANTI-TUMOR ACTIVITY, TO ACHIEVE THIS OBJECTIVE, THE TOMATO ORTHOLOGS OF FLAVO/ANTHOCYANIN REGULATORY GENES ROSEA1, DELILA AND MYB12 WILL BE ASSEMBLED SEAMLESSLY UNDER THE CONTROL OF TOMATO FRUIT/RIPENING SPECIFIC PROMOTERS IN ALL-TOMATO SELECTION MARKER-FREE BINARY VECTORS CONTAINING ENDOGENOUS P-DNA BORDER SEQUENCES, THE SECOND PROPOSED PRODUCT IS AN IMPROVED VERSION OF THE TOMATO-MADE HUMAN ANTI-ROTAVIRUS IMMUNOGLOBULIN A (IGA), DEVELOPED EARLIER IN OUR GROUP (BIO2008-01525), THE ORAL PROPHYLACTIC POTENTIAL OF THE CURRENT MONOCLONAL VERSION WILL BE EXPANDED BY ASSEMBLING NEW VARIABLE REGIONS TO THE BASIC IGA FRAMEWORK, THUS ENABLING A POLYCLONAL FORMULATION, THE CONSTRUCTION OF SECRETORY IGA COMPLEXES WILL BE ALSO FACILITATED BY THE MULTIGENE ASSEMBLING TOOLS,TO HELP ACHIEVING PROJECT GOALS, WE WILL DEVELOP A STANDARDIZED COLLECTION OF INTERCHANGEABLE TOMATO GENETIC PARTS (SO CALLED SOL-BRICKS, INCLUDING PROMOTERS, CDS, P-DNAS, ETC), THE SOL-BRICK COLLECTION, INSPIRED IN SYNTHETIC BIOLOGY STANDARDS, WILL BE MADE EASY-TO-ENSEMBLE IN AN INTERCHANGEABLE, SEMI-IDEMPOTENT AND SEAMLESS FASHION BY THE ADDITION OF FLANKING RECOGNITION SITES OF TYPE IIS RESTRICTION ENDONUCLEASES, THE CONSTRUCTION OF THE SOL-BRICK COLLECTION WILL FACILITATE MULTIGENE ENGINEERING AND WILL CONSTITUTE A FIRST STEP TOWARDS ENABLING SYNTHETIC BIOLOGY IN SOLANACEAE

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