Array tagged single cell gene expression by parallel linear RNA amplification an...
Array tagged single cell gene expression by parallel linear RNA amplification and sequencing
In many biomedical research and clinical applications it would be tremendously useful to know the gene expression profile of each and every cell in a sample, be it a blood sample or tumor. At present, the most advanced single-cell...
ver más
¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video
Proyectos interesantes
TIN2009-14057-C03-02
PLATAFORMA DE DESARROLLO E INTEGRACION DE TECNICAS INFORMADA...
115K€
Cerrado
PTQ2021-012221
Implementación de la tecnología de NGS de single cell RNA-se...
83K€
Cerrado
EQC2019-005885-P
Desarrollo de aplicaciones de análisis genómico y transcript...
182K€
Cerrado
UNSA10-3E-749
Servicio de análisis de expresión génica y genotipado median...
61K€
Cerrado
PTQ-10-04257
Diseño e implementación de métodos de Análisis de Datos expe...
61K€
Cerrado
PTQ-08-03-07929
Desarrollo de una Herramienta de Análisis de Vías de Señaliz...
50K€
Cerrado
Información proyecto ARRAY SEQ
Duración del proyecto: 18 meses
Fecha Inicio: 2015-08-18
Fecha Fin: 2017-02-28
Descripción del proyecto
In many biomedical research and clinical applications it would be tremendously useful to know the gene expression profile of each and every cell in a sample, be it a blood sample or tumor. At present, the most advanced single-cell technologies are limited to a few thousand cells by a laborious and expensive approach. We have invented a method allowing the determination of the transcriptomes of millions of cells in parallel, using array-based technique for tagging single cells. The protocol combines our previously published protocol for single cell transcriptomics – CEL-Seq – with a new membrane based system for capturing single cells and a DNA microarray for differentially tagging each cell in the membrane. If further developed into a commercial platform, our method could have tremendous impact on clinical and research transcriptomics. Our method requires no expensive equipment, low amounts of reagents and little hands-on, making it unlike any available protocol for single cell analysis. Our method also has great versatility as it can be used for analyzing up to a million cells, but can also be easily scaled down to several hundreds, promising to make it the state of the art protocol for any lab interested in single cell biology. Our method thus represents a game-changer because it completely reinvents the scale under which cells can be examined – affordably and without a need for expensive instruments – by at least three orders of magnitude. The aim of this project is to establish a user-friendly platform for our method that could be commercially available in the coming years. The developed platform will facilitate a large-scale ability to query cells; the breadth of possible research and personal medicine applications is unimaginable at present.