UNROOT
Financiado
Understanding the role and biogenesis of transfer RNA-derived small RNAs in plan...
This proposal aims to explore the precise mechanism, overall impact and complete population of the small RNAs derived of transfer RNAs (tsRNAs) in plants. The proposed research would be carried out in a laboratory that specializes...
This proposal aims to explore the precise mechanism, overall impact and complete population of the small RNAs derived of transfer RNAs (tsRNAs) in plants. The proposed research would be carried out in a laboratory that specializes in RNA regulation and that made substantial contributions to the plant tRNA-derived sRNA field (Dr. German Martinez). Although it has been described that these molecules are highly expressed in stress conditions, we still lack a clear understanding of the biogenesis pathways that lead to tsRNA accumulation, as well as of the array of biological processes they influence and their mechanism of action. Additionally, because of the widespread presence of RNA modifications in these molecules, the unbiased genome-wide quantification is not possible with the conventional sequencing technologies. Therefore, these molecules represent a largely uncharacterized stress-responsive regulatory network in plants, and thus an exciting source of potential applications for agriculture. The experimental approach of this project will use a model plant (Arabidopsis thaliana) in order to characterize the tsRNA population and evaluate the importance of this regulatory layer under viral stress. First, a method to compare the accumulation profiles of tsRNAs based on the enzymatic removal of RNA modifications will be implemented. Second, the biogenesis pathway of tRNA-derived sRNAs will be studied using mutant plants, and how this affects viral infection will be evaluated with these mutant plants. Third, the genes targeted by tRNA-derived sRNAs will be identified using a combined approach based on the immunoprecipitation of crosslinked AGO proteins and ribosome profiling that determines the translation rate. Overall, the data that this project would obtain has the potential to shift our understanding of tsRNAs and provide cutting-edge applications for agriculture. Thus, the completion of this project would provide me a considerable professional maturity.
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Duración del proyecto: 37 meses
Fecha Inicio: 2023-04-27
Fecha Fin: 2026-05-31
Fecha Fin: 2026-05-31
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-04-27
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2022-PF-01-01:
MSCA Postdoctoral Fellowships 2022
Cerrada
hace 2 años
Presupuesto
El presupuesto total del proyecto asciende a
207K€
Líder del proyecto
SVERIGES LANTBRUKSUNIVERSITET
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5