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FutureAgriculture

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Transforming the future of agriculture through synthetic photorespiration

For a new green revolution to feed the continually increasing population, agriculture productivity will have to be significantly improved. Photorespiration represents a big challenge in this respect, because it dissipates energy a... ver más

31/03/2021

MPG

5M€

Presupuesto del proyecto: 5M€

Líder del proyecto

MAXPLANCKGESELLSCHAFT ZUR FORDERUNG DER WISSE... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Ver 6 Participantes

Financiación concedida El organismo H2020 notifico la concesión del proyecto el día 2021-03-31

FETOPEN-RIA-2014-2015: FET-Open research projects Scope:Specific challenge: Supporting a large set of early stage, high risk visionary science and tec...

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6 Participantes

MPG

1.93M€ | Lider

MOVILIDAD INGENIERIA SOFTWAR...

253.59K€ | Participante

IMPERIAL COLLEGE OF SCIENCE...

955.22K€ | Participante

WEIZMANN

875.89K€ | Participante

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Duración del proyecto: 64 meses Fecha Inicio: 2015-11-11
Fecha Fin: 2021-03-31

Líder del proyecto

MAXPLANCKGESELLSCHAFT ZUR FORDERUNG DER WISSE... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 5M€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto For a new green revolution to feed the continually increasing population, agriculture productivity will have to be significantly improved. Photorespiration represents a big challenge in this respect, because it dissipates energy and leads to the futile loss of CO2, thereby limiting plant growth yield. Implementing an efficient metabolic bypass for photorespiration can therefore increase the photosynthetic efficiency of many cultivated crops. Several such routes were previously proposed. However, these routes were limited to existing enzymes and pathways and provided only partial improvement. Here, we propose a radically different approach: to engineer entirely novel CO2-neutral or CO2-positive photorespiration bypasses based on novel enzyme chemistry that support significantly higher agricultural yields. These bypass routes could support 60% higher biomass yield per turn of the Calvin Cycle and >30% higher yield per ATP. Our project innovatively integrates different research disciplines and combines academic research with industrial implementation. In silico studies will integrate biochemical logic and pathway modelling to explore all possible photorespiration pathways and identify the most efficient routes. In vitro research will establish novel enzyme functions via enzyme engineering and directed evolution. Full pathways will be reconstituted and optimized in vitro using a novel mass spectrometry based platform. High in vivo activity will be selected by implementing the pathways in engineered E. coli strains. Enhanced photosynthetic efficiency will be demonstrated in cyanobacteria expressing the synthetic pathways. Finally, the most promising synthetic pathways will be implemented in higher plants and growth phenotypes will be monitored. The proposed project comprises a significant advance in synthetic biology – applying biochemical principles to modify the very core of carbon metabolism with synthetic pathways that carry multiple novel enzymatic functions.

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