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Teaching Lytic Polysaccharide Monooxygenases to do Cytochrome P450 Catalysis

"Lytic polysaccharide monooxygenases (LPMO) and cytochrome P450 (CYP) are copper- and iron-dependent, respectively, enzymatic systems that perform regio- and stereospecific oxidation of non-activated hydrocarbons in Nature. To con... ver más

31/05/2025

HIOF

3M€

Presupuesto del proyecto: 3M€

Líder del proyecto

HOGSKOLEN I OSTFOLD No se ha especificado una descripción o un objeto social para esta compañía.

Ver 4 Participantes

Financiación concedida El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2022-02-17

Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:

HORIZON-EIC-2021-PATHFINDEROPEN-01-01: EIC Pathfinder Open 2021

I+D

Cerrada hace 3 años

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4 Participantes

HIOF

1.16M€ | Lider

POLITECHNIKA SLASKA

685.75K€ | Participante

TU GRAZ

781.95K€ | Participante

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Duración del proyecto: 39 meses Fecha Inicio: 2022-02-17
Fecha Fin: 2025-05-31

Líder del proyecto

HOGSKOLEN I OSTFOLD

Presupuesto del proyecto 3M€

Descripción del proyecto "Lytic polysaccharide monooxygenases (LPMO) and cytochrome P450 (CYP) are copper- and iron-dependent, respectively, enzymatic systems that perform regio- and stereospecific oxidation of non-activated hydrocarbons in Nature. To control such reactions in modern industry and biotechnology is of utmost importance in creating products of value such as secondgeneration bioethanol and products of value for i.e. the pharmaceutical industry. Due to the major drawbacks of using CYPs, including their partially membrane bound nature and the requirement of a reductase in combination with reducing agents such as NAD(P)H to transfer electrons to the active site for oxygen activation, it is highly desirable to develop new type of catalyst that can perform the same type of reactions. An attractive alternative strategy is to engineer LPMOs to perform CYP catalysis. LPMOs are small, robust, easy to produce in large scale, and rigid water-soluble proteins with a plethora of electron donors. The extended, flat LPMO surface, with huge natural sequence variation and thus, likely, mutability, provides a fantastic scaffold for engineering access to the active site as well as substrate affinity. We propose to use LPMOs engineered to accommodate typical CYP substrates and immobilize this on solid supports to provide confinement necessary in bringing the oxygen species together with the C-H bond to be oxidized in a tailored, ""closed"" environment. Moreover, the rate of LPMO catalysis can be greatly enhanced compared to traditional CYP catalysis by the addition H2O2 in the presence of low, priming concentrations of an external reductant to achieve efficiency constants (kcat/Km) in the order of 106 M-1s-1, which is typical for peroxygenases. The proposed ground-breaking research fits excellently well with the work program ""Future and Emerging Technologies"" where the goal is to challenge current thinking."

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