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SinFonia

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Synthetic biology guided engineering of Pseudomonas putida for biofluorination

Nature has hardly evolved biochemical reactions involving fluorine (F), the most abundant halogen on Earth. Organic compounds containing F (fluorochemicals) are, however, extremely relevant from an industrial point of view. Fluoro... ver más

31/08/2023

TECHNICAL UNIVERSI...

8M€

Presupuesto del proyecto: 8M€

Líder del proyecto

DANMARKS TEKNISKE UNIVERSITET No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Ver 14 Participantes

Financiación concedida El organismo H2020 notifico la concesión del proyecto el día 2023-08-31

BIOTEC-03-2018: Synthetic biology to expand diversi... Specific Challenge:For years, industries have relied upon living organisms as a source of compounds...

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Este proyecto no cuenta con búsquedas de partenariado abiertas en este momento.

Información adicional privada

No hay información privada compartida para este proyecto. Habla con el coordinador.

14 Participantes

TECHNICAL UNIVERSITY OF DENM...

2.39M€ | Lider

MONTAJES ELECTRONICOS DORCAS

234.43K€ | Participante

CHEMOURS

N.D. | Participante

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Duración del proyecto: 56 meses Fecha Inicio: 2018-12-14
Fecha Fin: 2023-08-31

Líder del proyecto

DANMARKS TEKNISKE UNIVERSITET No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 8M€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto Nature has hardly evolved biochemical reactions involving fluorine (F), the most abundant halogen on Earth. Organic compounds containing F (fluorochemicals) are, however, extremely relevant from an industrial point of view. Fluoropolymers are the main fluorochemicals in the market worldwide, and are exclusively synthesized using chemical methods. Moreover, current fluorination technologies usually involve corrosive and toxic reagents that have a negative impact on the environment. Designing sustainable bioprocesses based on alternative and safer fluorinating agents from renewable substrates is thus a long-sought-after, yet unfulfilled goal. SinFonia proposes to engineer the metabolically-versatile bacterium Pseudomonas putida to execute biofluorinations for generating novel fluoropolymers from renewable substrates. P. putida KT2440, a non-pathogenic soil bacterium, serves as an ideal microbial platform for F-dependent biochemical reactions due to its extraordinary resistance to harsh and stressful operating conditions. SinFonia will exploit natural selection to enhance bioproduction through a smart strain engineering approach in which bacterial growth will be coupled to biofluorination. Our target compounds are a whole family of fluorinated polyesters with enhanced physicochemical and material properties, with uses as self-cleaning surfaces, low-surface-energy coatings, bio-based lubricants, membranes for fuel cells, and anti-fouling materials. The versatile P. putida strains engineered during the project can be easily adapted to synthesize other added-value fluorochemicals. Unlike chemical processes, the source of F in our system will be NaF, an inexpensive and safe salt, and sugars as the main carbon source. In-depth analysis of all the environmental and economic benefits of the new fluorination technology, and interactive communication of social benefits associated with target products, are essential components of SinFonia.

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