FuncAmyloid
Financiado
Structure, Function and Regulation of Antimicrobial and Virulent Amyloids at Hig...
Structure, Function and Regulation of Antimicrobial and Virulent Amyloids at High-resolution
Self-assembly of proteins and peptides into amyloid fibrils produced across kingdoms of life is associated with antimicrobial activity, microbial pathogenicity, and a wide range of diseases. The correlation of fibrillation and mor...
Self-assembly of proteins and peptides into amyloid fibrils produced across kingdoms of life is associated with antimicrobial activity, microbial pathogenicity, and a wide range of diseases. The correlation of fibrillation and morphology to function is poorly understood, and high-resolution structural information and mechanistic models are lacking. Our lab pioneered the atomic-level analysis of bacterial amyloids and eukaryotic functional fibrils involved in cytotoxicity, biofilm structuring, and antibacterial activity. We revealed novel morphologies extending beyond canonical amyloid cross-β structures of tightly mated β-sheets, to include, for example, cross-α fibrils composed on amphipathic α-helices. In addition, we exposed a unique lipid-induced cross-α/β secondary structure switch in fibrils of the same sequence. Here we investigate amyloid fibrils which serve as key virulence determinants in S. aureus and Pseudomonas acting as cytotoxins and in biofilm scaffolding, and as antimicrobials produced across different species. We will leverage the knowledge and expertise, and newly emerging methods in electron, light and force microscopy, to understand how fibrillation propensity, fibril morphology and structural switches are connected to function, membrane interactions and toxicity mechanisms at high resolution. The findings are expected to identify structural features that underlie the formation, regulation, and activity of these fibrils, providing advantages in specific environments. Understanding these structure-function relationships will help to clarify the link between amyloid formation and antimicrobial activity. We will use the insights gained from these studies for the rational design of antimicrobial peptides and small molecules targeting virulent determinants towards potential applications in the management of infectious diseases. Our findings on functional amyloids can overall advance life, material, medical and environmental sciences.
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Duración del proyecto: 65 meses
Fecha Inicio: 2023-03-31
Fecha Fin: 2028-09-30
Fecha Fin: 2028-09-30
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-03-31
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
ERC-2022-COG:
ERC CONSOLIDATOR GRANTS
Cerrada
hace 2 años
Presupuesto
El presupuesto total del proyecto asciende a
2M€
Líder del proyecto
DEUTSCHES ELEKTRONENSYNCHROTRON DESY
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5