Hola,
¿eres nuevo aquí?

Regístrate gratis y conecta tu empresa con financiación pública, partners y proyectos.

Tengo cuenta

Regístrate

Ver video

P-TYPE ATPASES

Financiado

Cerrado

Structural studies of P type ATPases and their characterization in native membra...

Structural studies of P type ATPases and their characterization in native membranes P-type ATPases are cell-membrane pumps that are of fundamental importance in eukaryotes. The ion gradients created by Ca2+-ATPases or Na+,K+-ATPases are involved in signalling, maintaining the membrane potential as well as seconda... ver más

31/12/2009

204K€

Presupuesto del proyecto: 204K€

Líder del proyecto

AARHUS UNIVERSITET No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo FP7 notifico la concesión del proyecto el día 2009-12-31 No tenemos la información de la convocatoria

0% 100%

Información adicional privada

No hay información privada compartida para este proyecto. Habla con el coordinador.

1 Participantes

0.00€ | Lider

Proyectos interesantes

MPFP

Medical Technol...

Applied Physics

309K€ Cerrado

VISUALTRANSPORT

Medical Technol...

Applied Physics

100K€ Cerrado

ITrans

Medical Technol...

Applied Physics

63K€ Cerrado

ANOBEST

Medical Technol...

Applied Physics

2M€ Cerrado

ChemiLUCA

Medical Technol...

Applied Physics

188K€ Cerrado

BFU2008-04188-C03-02

Medical Technol...

Applied Physics

121K€ Cerrado

Conecta tu I+D

¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video

Líder del proyecto

AARHUS UNIVERSITET No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 204K€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto P-type ATPases are cell-membrane pumps that are of fundamental importance in eukaryotes. The ion gradients created by Ca2+-ATPases or Na+,K+-ATPases are involved in signalling, maintaining the membrane potential as well as secondary active transport. Despite the recent progress in membrane protein crystallography, the lack of detailed structural information currently prevents a better understanding of function and specificity of cation pumps. One specific aim of this proposed research is to determine the structure of the plasma-membrane Ca2+-ATPase (PMCA) and to understand its regulatory mechanism. The autoinhibited PMCA will be recombinantly expressed in yeast, purified and crystallized. In addition, it is also planned to determine the structure of the activated complex consisting of PMCA and calcium-bound calmodulin (Ca2+-CaM). In order to deepen our understanding of cation pumps in their native environment, I further propose to investigate Na+,K+-ATPases in native membranes using a multi-technique approach. First, I will analyze complexes of Na+,K+-ATPase isolated from native membranes by mass spectrometry to identify peripheral proteins. In the next step, I plan to assemble those identified multi-protein complexes in vitro and structurally characterize them by cryo-EM. Finally, I plan to use AFM to investigate the oligomeric arrangement of Na+,K+-ATPases in native membranes. This multi-disciplinary project will hopefully expand our knowledge of cation pumps by putting atomic details in the context of the native environment and will concurrently allow me to gain experience in membrane protein crystallography that will complement my expertise in biophysical methods.

Conecta tu I+D

Entra hoy

¿Olvidé mi contraseña?

Financiación

Empresas

CTIs/Universidades

Proyectos

Investigadores