Hola,
¿eres nuevo aquí?

Regístrate gratis y conecta tu empresa con financiación pública, partners y proyectos.

Tengo cuenta

Regístrate

Ver video

FINDR

Financiado

Spatiotemporal characterization of extrachromosomal DNA transcription and its ke...

Spatiotemporal characterization of extrachromosomal DNA transcription and its key regulators in cancer cells Extrachromosomal circular DNA (ecDNA) present in cancer cells stochastically amplifies oncogenes and drug resistance genes independent from the core genome and thereby contributes to tumor adaptability and heterogeneity. While ecD... ver más

31/03/2026

PrinsesMaximaCentr...

188K€

Presupuesto del proyecto: 188K€

Líder del proyecto

PRINSES MAXIMA CENTRUM VOOR KINDERONCOLOGIE B... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Financiación concedida El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2024-03-11

HORIZON-MSCA-2023-PF-01-01: MSCA Postdoctoral Fellowships 2023 ExpectedOutcome:Project results are expected to contribute to the following outcomes:

I+D

Cerrada hace 1 año

0% 100% 100%

Información adicional privada

No hay información privada compartida para este proyecto. Habla con el coordinador.

1 Participantes

PrinsesMaximaCentrumvoorKind...

187.62K€ | Lider

Conecta tu I+D

¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video

Proyectos interesantes

CancerCirculome Circular DNA driven cancer genome remodeling 1M€ Cerrado

RTI2018-102204-B-I00 MECANISMOS DE RESISTENCIA A TERAPIAS ANTITUMORALES 375K€ Cerrado

BFU2013-44343-P BASES MOLECULARES DE LA FORMACION DE TRANSLOCACIONES CROMOSO... 109K€ Cerrado

DARC MATTER Dynamics of Adaptation and Resistance in Cancer: MApping and... 2M€ Cerrado

SAF2017-87811-R MUTACIONES QUE AFECTAN A LA MADURACION DEL RNA Y MUTACIONES... 200K€ Cerrado

PID2020-116003GB-I00 GENOMICA AVANZADA PARA EL ESTUDIO DE LA INTEGRIDAD DE CROMOS... 272K€ Cerrado

Duración del proyecto: 24 meses Fecha Inicio: 2024-03-11
Fecha Fin: 2026-03-31

Líder del proyecto

PRINSES MAXIMA CENTRUM VOOR KINDERONCOLOGIE B... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 188K€

Descripción del proyecto Extrachromosomal circular DNA (ecDNA) present in cancer cells stochastically amplifies oncogenes and drug resistance genes independent from the core genome and thereby contributes to tumor adaptability and heterogeneity. While ecDNA prevalence, diversity, and biological traits have been studied, we lack insights into their expression regulation. It is unclear how it differs from chromosomal genes and how it is linked to the non-random and dynamic nuclear localization patterns observed for many ecDNA. Moreover, we are unaware of the proteins that participate in interactions with nuclear compartments to control ecDNA transcription. Most studies using sequencing and FISH methods fall short in describing these dynamic processes. However, understanding their implications is vital for designing therapies that target ecDNA expression as a whole instead of targeting individual oncogenes. To advance our knowledge, we need tools to map ecDNA transcription at high temporal and spatial resolution and systematic methods to screen for associated proteins. The aim of this project is to identify crucial overarching mechanisms and players of ecDNA transcription through an interdisciplinary approach. The Medema group has devised protocols to induce extrachromosomal amplification of drug resistance genes in diverse cellular backgrounds and genetically tag them for imaging and perturbation. The present study will build on this work to for the first time reveal the spatiotemporal co-regulation of ecDNA transcription and localization using innovative imaging reporters for measurements in single live cells and use unbiased proteomic profiling to map factors that interact with ecDNA and regulate their expression. Besides addressing outstanding questions in the field, our study will create unique cell models and datasets as a basis for future studies. Our results will furthermore inform the design of more effective treatments for the many cancer patients affected in Europe and worldwide.

Conecta tu I+D

Entra hoy

¿Olvidé mi contraseña?

Financiación

Empresas

CTIs/Universidades

Proyectos

Investigadores