Innovating Works

LIPMETIN-sURFing

Financiado
Small open reading frames smORF as novel modulators of disorders of dietary ex...
Dietary excess is the major origin of cardiovascular diseases and remains the leading cause of mortality. Due to difficulties with bioinformatics detection and experimental analysis, along with their sheer numbers, small open read... Dietary excess is the major origin of cardiovascular diseases and remains the leading cause of mortality. Due to difficulties with bioinformatics detection and experimental analysis, along with their sheer numbers, small open reading frames (smORF) –of fewer than 100 amino acids– have been largely disregarded. Advances in bioinformatics, RNA-sequencing (ribosome profiling), biochemical (peptidomics) and functional (genetic) screens, suggest that a large amount of smORF are transcribed and sometimes widely conserved. However, how much of these smORF-encoded peptides or small proteins (microproteins (MPs)) are translated into a functional peptide is largely unknown. Moreover, whether food bioactive components modulate the expression of MPs remains complete obscure. Thus, the main goal of this project is to search for MPs that regulate lipid metabolism in the intestine in response to dietary excess. The intellectual merits of this proposal are threefold. First, it will use cutting-edge technology to search for novel functional MPs. Second, it will address a new group of novel potential players not previously addressed in lipid metabolism, providing a novel insight into disease mechanism and innovative therapeutic strategies. Last, by testing two common food bioactive components from our healthy Mediterranean diet, we will open up a completely new field of research in the area of nutrition and health. The research approach will involve (i) a genome-wide peptidomic screen and ribosome profiling analysis of lipid-induced intestinal MPs, (ii) the use of in vivo and in vitro biochemical approximations to validate the function of the selected intestinal peptide, and (iii) the use of dietary components hydroxytyrosol and the omega-3 polyunsaturated fatty acid DHA as proof of concept that MPs can be modulated by our diet food bioactive. This project is framed in the Food & Healthy Diet and Biotechnology research area in the European Union's Framework Programme HORIZON 2020 ver más
05/09/2021
IMDEA ALIMENTACIÓN
257K€
Duración del proyecto: 53 meses Fecha Inicio: 2017-03-17
Fecha Fin: 2021-09-05

Línea de financiación: concedida

El organismo H2020 notifico la concesión del proyecto el día 2021-09-05
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
MSCA-IF-2016: Individual Fellowships
Cerrada hace 8 años
Presupuesto El presupuesto total del proyecto asciende a 257K€
Líder del proyecto
IMDEA ALIMENTACIÓN No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5 260K