Innovating Works

MultiCloneSeq

Financiado
Single-Clone Multi-omics Sequencing for Cancer Diagnosis
Cancer led to one in six death worlds wildly. By nature, cancer is characterized by abnormal and uncontrolled cellular growth caused primarily by genetic mutations. Despite of the high cellular heterogeneity, only a small number o... Cancer led to one in six death worlds wildly. By nature, cancer is characterized by abnormal and uncontrolled cellular growth caused primarily by genetic mutations. Despite of the high cellular heterogeneity, only a small number of somatic mutations shown to be directly associated with tumorigenesis, hence driving cancer growth. The first hurdle in the cancer diagnosis is to identify these rare populations that have the potential to develop drug resistance. A second hurdle is to be able to associate mutations in the genetic level to the RNA expression level, which will improve the chance of pinpointing a specific mutation with the relevant activity. Therefore, it is of crucial importance to develop a single-cell multi-omics sequencing tool to simultaneously study the heterogeneity of expression and DNA-based regulation of cancer-associated genes. Recently, several multi-omics tools have emerged that allow for the interrogation of both gene expression and open chromatin regions. However, there are some notable limitations constrain the capability and applicability of these tools: 1) The expensive and disposable apparatuses and reagents of these methods is extremely expensive, and thus limits their extensive application and potential as a diagnosis tool; 2) The transcriptome derived from all these technologies only captures the 3 ' RNA termini of polyadenylated transcripts. It excludes the capture of non-polyadenylated transcripts which plays a vital role in cancer development, and the interrogation of somatic mutation from full length RNA. (3) The low input of single-cell material leads to the sparse data. To overcome these hurdles, our novel technology is based on three methods that we have developed leveraging the power of microfluidics: 1. single-clone sequencing, 2.Full-length RNA, and 3. A cost efffective open chromatin extraction method. MultiCloneSeq will be the first method providing low-cost and full-scale multiomic profiling with high resolution. ver más
31/03/2025
THE HEBREW UNIVERS...
150K€
Perfil tecnológico estimado
Duración del proyecto: 18 meses Fecha Inicio: 2023-09-26
Fecha Fin: 2025-03-31

Línea de financiación: concedida

El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-09-26
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
ERC-2023-POC: ERC PROOF OF CONCEPT GRANTS
Cerrada hace 1 año
Presupuesto El presupuesto total del proyecto asciende a 150K€
Líder del proyecto
THE HEBREW UNIVERSITY OF JERUSALEM No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5