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SMACK

Financiado

Single molecule tracking for live cell protein synthesis kinetics

This project aims to map the determinants of efficient synthesis, folding, and targeting of proteins in living bacterial cells, and to understand how antibiotic drugs inhibit these events. Our findings will be used to form a quant... ver más

31/12/2025

2M€

Presupuesto del proyecto: 2M€

Líder del proyecto

UPPSALA UNIVERSITET No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Ver 2 Participantes

Financiación concedida El organismo H2020 notifico la concesión del proyecto el día 2020-09-09

Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:

ERC-2020-STG: ERC STARTING GRANTS

I+D

Cerrada hace 5 años

0% 100%

2 Participantes

1.65M€ | Lider

AUTO STAMP

455.47K€ | Participante

Últimas noticias

29-11-2024: IDAE En las últimas 48 horas el Organismo IDAE ha otorgado 4 concesiones

29-11-2024: ECE En las últimas 48 horas el Organismo ECE ha otorgado 2 concesiones

29-11-2024: CMVAMADRID En las últimas 48 horas el Organismo CMVAMADRID ha otorgado 37 concesiones

Duración del proyecto: 63 meses Fecha Inicio: 2020-09-09
Fecha Fin: 2025-12-31

Líder del proyecto

UPPSALA UNIVERSITET

TRL 4-5

Presupuesto del proyecto 2M€

Descripción del proyecto This project aims to map the determinants of efficient synthesis, folding, and targeting of proteins in living bacterial cells, and to understand how antibiotic drugs inhibit these events. Our findings will be used to form a quantitative description of how the mRNA sequence ultimately determines how, where, and when a polypeptide should fold, which will help to design better E. coli-based production systems for recombinant proteins; both protein folding and targeting are currently severe bottlenecks. Further, by studying the effect of clinically relevant antibiotic drugs directly on ongoing protein synthesis, we will learn how the inhibitory action on the molecular level is translated into the response on cell and population level. This knowledge will help to improve the use of current drugs, as well as to provide clues regarding how new drugs should be designed. To reach our goals, we will use and further develop our recently published fluorescence-based experimental and analytical system for live-cell single-molecule analysis of protein synthesis kinetics. Particularly, we will develop orthogonal translation systems to measure protein synthesis kinetics on defined mRNAs in an unperturbed cell background. We will also label and study key components of the universal pathway for Signal Recognition Particle-mediated cotranslational targeting of peptides to the membrane-bound peptide translocation complexes. In these applications we use small organic dyes for site-specific labeling of molecules, which allow recording of long diffusion trajectories inside cells. These trajectories are then fed to our machine-learning algorithms to deduce the binding kinetics. With an increasing demand for therapeutic proteins, such as monoclonal antibodies for cancer treatment, there is an urgent need for microbial-based systems for cost-effective production of recombinant proteins. With our unique experimental and analytical system, we have the opportunity to aid in this development.

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