Innovating Works

BACTOSHAPE

Financiado
Single cell biophysics of bacterial cell shape
In bacteria, the rigid external cell wall (CW) and the intracellular actin-like (MreB) cytoskeleton are major determinants of cell shape. Synthesis and chemical composition of the CW, a three dimensional polymer network that is on... In bacteria, the rigid external cell wall (CW) and the intracellular actin-like (MreB) cytoskeleton are major determinants of cell shape. Synthesis and chemical composition of the CW, a three dimensional polymer network that is one of the most prominent targets for antibiotics, are well understood. However, despite decades of study, little is known about the complex CW ultrastructure and the molecular mechanisms that control cell shape in time and space. MreB homologues assemble into dynamic membrane-associated structures thought to control shape by serving as organizers for the movement and assembly of macromolecular machineries responsible for CW biogenesis. However, the mechanistic details used by the MreB cytoskeleton to fulfill this role remain to be elucidated. We will combine powerful genetic tools available in the model Gram-positive bacterium Bacillus subtilis with modern high-resolution fluorescence microscopy techniques and atomic force microscopy (AFM) to study the role of the MreB cytoskeleton and CW synthesis proteins in cell shape determination and maintenance. Additionally, the role of mechanical forces in the control of CW organization will be evaluated. ver más
31/03/2017
INRAE
185K€
Duración del proyecto: 24 meses Fecha Inicio: 2015-03-25
Fecha Fin: 2017-03-31

Línea de financiación: concedida

El organismo H2020 notifico la concesión del proyecto el día 2017-03-31
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
MSCA-IF-2014-EF: Marie Skłodowska-Curie Individual Fellowships (IF-EF)
Cerrada hace 10 años
Presupuesto El presupuesto total del proyecto asciende a 185K€
Líder del proyecto
INSTITUT NATIONAL DE RECHERCHE POUR LAGRICULT... No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5