PIPContactSite
Financiado
Roles of maize PIP2 aquaporins in the structural organization of the cell
Plasma membrane (PM) Intrinsic Proteins (PIPs) are aquaporins that facilitate the diffusion of water through cell membranes. By regulating PIP activity, plant cells adjust water permeability rapidly and reversibly. PIP PM lateral...
Plasma membrane (PM) Intrinsic Proteins (PIPs) are aquaporins that facilitate the diffusion of water through cell membranes. By regulating PIP activity, plant cells adjust water permeability rapidly and reversibly. PIP PM lateral diffusion is affected by the cell wall and actin. Also, PM-located PIPs interact with VAP27s at the endoplasmic reticulum (ER)–PM contact sites (EPCS). Evidence suggests that PIPs get organized in interaction with other cellular structures. However, the functional relevance of these interactions has not been extensively studied. The PIPContactSites project's main goal is to understand whether ZmPIP2s mediate PM interaction with other cellular structures (ER, cytoskeleton) and elucidate the physiological relevance of the contact sites under salt stress conditions. Our hypothesis is that PIP2 interactions on EPCS and with the cytoskeleton facilitate an efficient response to stimuli by participating in the structural organization of the cell. Our strategy integrates cutting-edge technologies for genome edition (CRISPR/Cas9) and real-time monitoring of interactions (split Fluorescence-Activating and absorption Shifting Tag) with advanced microscopy technologies (i.e., confocal super-resolution and Correlative Light and Electron Microscopy). The originality of this project is to consider PIPs as multifunctional proteins: channels and scaffold proteins. The MSCA fellowship, the great intellectual environment, the infrastructure and the administrative support at the LIBST-UCLouvain, the research and project managing experience of Prof. Chaumont, and my background in molecular biology will synergistically promote the success of the project. The host group will benefit from hosting an independent, creative, and highly skilled scientist. And I will gain experience and networking opportunities to shape my long-term objective of becoming a group leader.
ver más
Duración del proyecto: 24 meses
Fecha Inicio: 2022-05-17
Fecha Fin: 2024-05-31
Fecha Fin: 2024-05-31
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2024-05-31
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2021-PF-01-01:
MSCA Postdoctoral Fellowships 2021
Cerrada
hace 3 años
Presupuesto
El presupuesto total del proyecto asciende a
192K€
Líder del proyecto
UNIVERSITE CATHOLIQUE DE LOUVAIN
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5