Innovating Works

DormantMicrobes

Financiado
Revealing the function of dormant soil microorganisms and the cues for their awa...
Soils are considered the last scientific frontiers that harbor one of the most diverse microbial communities on Earth. It is hypothesized that this diversity allows for redundancy in microbial key processes, thereby ensuring ecosy... Soils are considered the last scientific frontiers that harbor one of the most diverse microbial communities on Earth. It is hypothesized that this diversity allows for redundancy in microbial key processes, thereby ensuring ecosystem stability. Much of this functional redundancy is embodied in non-active, dormant microorganisms that represent the ‘microbial seed bank’, which is characterized by a high number of low abundant taxa. Based on the recent theory of a ‘dynamic rank-abundance curve’, it is hypothesized that the rare dormant organisms can be recruited to participate in a given function upon resuscitation with environmental cue(s). In this project I will test this hypothesis on a level that matters for ecosystem processes – the functional level – by an innovative approach combining stable isotope probing (SIP) and sequencing with process-level and single-cell activity analysis. By testing 4 hypotheses, we will (1) reveal environmental cues that resuscitate dormant microorganisms involved in major soil functions and identify the activated microorganisms. The activity of the resuscitated communities will be analyzed at the process level, as well as at the single-cell by NanoSIMS, thereby allowing us to elucidate the impact of dormancy/resuscitation dynamics on targeted processes at the population and ecosystem level. (2) We will investigate the genetics of microbial dormancy-resuscitation strategies in a natural model environment for dormancy, an arid ecosystem, by metatranscriptome analysis of critical dormancy-resuscitation steps. (3) We will retrieve genomic information of primarily rare, but after resuscitation active, microorganisms involved in important soil processes, as they presumably contain so far unknown genomic potential. In summary, this project will generate essential knowledge on the stability of microbial key processes and on the diversity, the function and the genetics of the dormant majority in terrestrial ecosystems. ver más
30/04/2022
1M€
Duración del proyecto: 84 meses Fecha Inicio: 2015-04-22
Fecha Fin: 2022-04-30

Línea de financiación: concedida

El organismo H2020 notifico la concesión del proyecto el día 2022-04-30
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
ERC-StG-2014: ERC Starting Grant
Cerrada hace 10 años
Presupuesto El presupuesto total del proyecto asciende a 1M€
Líder del proyecto
UNIVERSITAT WIEN No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5