RESIST
Financiado
Receptor Ectodomain Shedding in Plant Immune Signal Transduction
Receptor ectodomain shedding (shedding) is a fundamental mechanism of the regulation of communication between mammalian cells. Shedding refers to the proteolytic cleavage of the extracellular portion of a membrane protein. This pr...
Receptor ectodomain shedding (shedding) is a fundamental mechanism of the regulation of communication between mammalian cells. Shedding refers to the proteolytic cleavage of the extracellular portion of a membrane protein. This process can inactivate the function of a receptor, generate extracellular signalling molecules and trigger the generation of intracellular protein forms involved in signalling. Shedding is best understood in mammals, with hundreds of shedding events reported, but also occurs in other animals and fungi. A few cases of shedding have been observed in plants. Membrane receptor like kinases (RLKs) constitute the main players of cell-to-cell communication in plants, with many of them serving as key immune receptors. RLK shedding has been reported in two occasions, pointing to an important regulatory function. Despite the essential functions of shedding in animals, the biological significance of RLK shedding in plants, and the fact that plant cells harbour many more receptors than their animal counterparts, shedding has yet to be studied systematically in plants. By reanalysis of large scale extracellular proteome datasets, I found evidence of widespread RLK shedding events in plants. However, this analysis could not reveal exact cleavage sites that are important for follow up functional studies. Trough RESIST, I seek to develop this new field of ectodomain shedding in plant biology and study the mechanisms of regulation and the consequences of plant RLK shedding. To do so, I aim to establish a platform for experimental identification of shedding events in plants by adapting cutting edge methods recently developed in medical sciences for precise identification of processed membrane proteins and released ectodomains in Nicotiana benthamiana. I will use this platform to analyse the plant sheddome (the repertoire of shed proteins) and its dynamics upon defence activation and to identify interesting shedding events for functional characterization.
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Duración del proyecto: 32 meses
Fecha Inicio: 2022-06-20
Fecha Fin: 2025-02-28
Fecha Fin: 2025-02-28
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2022-06-20
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2021-PF-01-01:
MSCA Postdoctoral Fellowships 2021
Cerrada
hace 3 años
Presupuesto
El presupuesto total del proyecto asciende a
190K€
Líder del proyecto
FORSCHUNGSZENTRUM JULICH GMBH
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5