Fecha
Inicio: 01/04/2015, Fin: 30/09/2016.
Objetivos
Objetivos del proyecto
The project aims to create a demo system for cost effective, non-invasive device for rapid detection of cystic fibrosis inhumans.The detection of human recessive diseases has been dominated by the use of fluorescent biomarkers, based on organicdyes, helping researchers to study and analyse gene expression, cell cycle, and enzymatic activity. Among severalproteolytic enzymes, trypsin has attracted much attention, as it is a target in the study of...
The project aims to create a demo system for cost effective, non-invasive device for rapid detection of cystic fibrosis inhumans.The detection of human recessive diseases has been dominated by the use of fluorescent biomarkers, based on organicdyes, helping researchers to study and analyse gene expression, cell cycle, and enzymatic activity. Among severalproteolytic enzymes, trypsin has attracted much attention, as it is a target in the study of various important human recessivediseases including, for example, cystic fibrosis (CF).We present herein two colour encoded silica nanospheres (2nanoSi) for the fluorescence quantitative ratiometricdetermination of cystic in humans. Current detection technologies for cystic fibrosis diagnosis are slow, costly and sufferfrom false positives. The 2nanoSi proved to be a fast (minutes), a single-step and with two times higher sensitivity than thestate-of-the-art biomarkers based sensors for cystic fibrosis, allowing the quantification of trypsin concentrations in a widerange (25-350 μg/L). Moreover, our approach can be used from the 4th day of life when the trypsin concentration is alreadythe same as in adults. Furthermore, as trypsin is directly related to the development of cystic fibrosis (CF), different humanphenotypes, i.e. normal (160-340 μg/L), CF homozygotic (0-90 μg/L), and CF heterozygotic (91-349 μg/L), respectively, canbe determined using our 2nanoSi nanospheres. We anticipate the 2nanoSi system to be a starting point for non-invasive,easy-to-use and cost effective ratiometric fluorescence biomarker for recessive genetic diseases alike human cystic fibrosis.
ver más
Ambito
Comunidad autónoma: Se buscaba un proyecto en cooperación con un partner de CCAA especificas.
Este proyecto fue tramitado con éxito!.