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ViDaMa

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Proving causality of liquid liquid phase separation for the acquisition of nucle...

Proving causality of liquid liquid phase separation for the acquisition of nuclear like functions by Giant Viruses Viral Factories Giant Viruses (GVs) encode thousands of ORFan genes but their study has been nearly impossible due to the lack of genetic tools for reverse genetics. The aims of ViDaMa and the tools I designed will shed light into this Viral prot... see more

31/08/2029

CNRS

1M€

Project Budget: 1M€

Project leader

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

PARTICIPATION DEPralty Sin fecha límite de participación.

Financing granted El organismo HORIZON EUROPE notifico la concesión del proyecto The day 2024-08-27

ERC-2024-STG: ERC STARTING GRANTS Scope:Objectives

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1 Participantes

CNRS

1.50M€ | Leader

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Project duration: 60 months Date Start: 2024-08-27
End date: 2029-08-31

Project leader

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE... No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Project Budget 1M€

participation deadline Sin fecha límite de participación.

Project description Giant Viruses (GVs) encode thousands of ORFan genes but their study has been nearly impossible due to the lack of genetic tools for reverse genetics. The aims of ViDaMa and the tools I designed will shed light into this Viral protein Dark Matter. Particularly, I will study how new genes from Mimivirus were engineered during evolution to acquire nuclear-like function for their Viral Factories (VFs). VFs physically separate viral DNA replication and transcription from translation and likely segregate DNA into active and silent. How these functions are accomplished remains elusive. I will demonstrate causality of liquid-liquid phase separation (LLPS) for the acquisition of nuclear-like functions of the Viral Factories (VFs) of Mimivirus. To do so, I will: 1) Generate genome-wide loss-of-function screens for the identification of gene function and proteome-wide localization of virtually all Mimivirus and its host Acanthamoeba proteins. 2) Combine biochemistry and cell biology to dissect the nature, functions and components of the VFs of Mimivirus. 3) Strip down VFs to their minimal components in order to utilize them to improve production efficiency and purity of mRNA during in vitro transcription. ViDaMA will empower the GVs scientific community with genome-wide and proteome-wide data of gene function and protein localization. It will transform the field from mostly descriptive to allow the dissection of the molecular mechanisms behind viral phenotypes. ViDaMa will also address the molecular mechanisms that allow the VFs to acquire nuclear-like functions, directly tackling the viral eukaryogenesis theory. The new classification of VFs as membrane-less organelles and the study of the molecular mechanism behind their biogenesis and functions will shed light into general mechanism of LLPS. The generation of in vitro VFs promises an optimization of in vitro transcription systems with tremendous impact on mRNA therapeutics at the development and production level.

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