G4invivo
Financiado
Probing intracellular folding and dynamics of telomeric DNA structures with sing...
Probing intracellular folding and dynamics of telomeric DNA structures with single molecule FRET
Telomeres, the ends of linear eukaryotic chromosomes, are one of the most versatile molecular machines of life. They perform a vital role in several fundamental processes in biology, as maintenance of genome integrity and the regu...
Telomeres, the ends of linear eukaryotic chromosomes, are one of the most versatile molecular machines of life. They perform a vital role in several fundamental processes in biology, as maintenance of genome integrity and the regulation of cell growth. Human telomeric DNA consists of thousands of tandem repeats of guanine-rich sequences, terminating the chromosomes. Under physiological conditions, such guanine-rich repeats are capable of adopting four stranded structures, called G-quadruplex DNA. Considerable research has been dedicated to an understanding of functions of telomeres, and during the past decades, much progress has been made towards identifying the underlying processes for G-quadruplex DNA isolated in vitro. However, most of our knowledge of conformation and mechanistic understanding of the balance between the structure and function of telomeric DNA inside cells has remained very limited, largely due to a lack of suitable methods. An opportunity to bridge this gap is single-molecule fluorescence microscopy in combination with Förster resonance energy transfer (FRET), a powerful technique that allows distances, distance distributions, and dynamics of individual biological molecules to be measured even in complex and heterogeneous environments, as cellular milieu.
The goal of this project is to employ such optical single-molecule techniques to perform a comprehensive biophysical analysis of the folding and dynamics of G-quadruplex DNA within live cells. This investigation will provide an idea of how accurately our current understanding of the folding and dynamics of G-quadruplex DNA in vitro describes the situation inside the living cell, and will, among other aspects, be crucial for understanding the biological functions of G-quadruplexes and in particular may stimulate the enhancement of the efficacy of the novel anticancer therapies, which use these structures as an active target for drug development.
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Duración del proyecto: 33 meses
Fecha Inicio: 2015-03-27
Fecha Fin: 2017-12-31
Fecha Fin: 2017-12-31
Línea de financiación: concedida
El organismo H2020 notifico la concesión del proyecto el día 2017-12-31
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
MSCA-IF-2014-EF:
Marie Skłodowska-Curie Individual Fellowships (IF-EF)
Cerrada
hace 10 años
Presupuesto
El presupuesto total del proyecto asciende a
212K€
Líder del proyecto
AARHUS UNIVERSITET
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5
769.98K€ |
Participante