SymPore
Financiado
Plasmodesmata Symplasmic pores for plant cell to cell communication
During evolution of multicellularity, cells differentiated to become specialized and interdependent. Multicellular organisms invented channels for nutrient exchange and communication between cells. Plants uniquely developed plasmo...
During evolution of multicellularity, cells differentiated to become specialized and interdependent. Multicellular organisms invented channels for nutrient exchange and communication between cells. Plants uniquely developed plasmodesmata, complex cell-cell connections traversing the cell wall. Roles ascribed to plasmodesmata include selective transport of signals, ions, metabolites, RNAs and proteins. Due to technical hurdles, composition, structure and regulation of plasmodesmatal conductance remain enigmatic. Genetic approaches to study plasmodesmata were hampered by lethality or redundancy. Novel technologies now set the stage for resolving roles of plasmodesmata in transport and signaling in an interdisciplinary approach. We will use proximity labeling proteomics to obtain plasmodesmatal composition, and PAINT and cryo electron tomography (cryoET) for near atomic structures. Models of plasmodesmata will be built from bottom up and top down approaches and combined with quantitative assessment of plasmodesmatal activity. Novel biosensor approaches together with knock down by genome editing will permit quantitation of transport of the diverse cargo. Single cell sequencing helps fine-tuning mutant selection and targeting of subtypes. Four labs will join forces: highly recognized experts in biophysics and cryoET (WB), advanced imaging and developmental signaling (RS), high-end proteomics and lipidomics (WS), and interactomics, transporters and cutting-edge biosensor technology (WF). We will iteratively address: (1) systematic quantitative identification of components, (2) their localization and dynamics, (3) structures and molecular building blocks of diverse plasmodesmatal types, and (4) transport and signaling mechanisms. We expect breakthrough discoveries and completely new understanding of plasmodesmatal function and evolution. Since plasmodesmata play key roles in nutrient allocation and virus spread, we lay the basis for novel biotech solutions in agriculture.
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Duración del proyecto: 76 meses
Fecha Inicio: 2020-11-13
Fecha Fin: 2027-03-31
Fecha Fin: 2027-03-31
Línea de financiación: concedida
El organismo H2020 notifico la concesión del proyecto el día 2020-11-13
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
ERC-2020-SyG:
ERC Synergy Grant
Cerrada
hace 5 años
Presupuesto
El presupuesto total del proyecto asciende a
11M€
Líder del proyecto
HEINRICH-HEINE-UNIVERSITAET DUESSELDORF
No se ha especificado una descripción o un objeto social para esta compañía.
370.00K€ |
Participante