On chip simultaneous intracellular recording and stimulation of electrical and b...
On chip simultaneous intracellular recording and stimulation of electrical and biochemical activities from hundreds of neurons
In the BRAINSTORM project, we aim to develop an integrated system enabling on-chip intracellular recordings from a relatively large cultured neuronal network combined with chemical sensing and intracellular delivery of biologicall...
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Descripción del proyecto
In the BRAINSTORM project, we aim to develop an integrated system enabling on-chip intracellular recordings from a relatively large cultured neuronal network combined with chemical sensing and intracellular delivery of biologically active molecules. To achieve tight coupling with the cell membrane and enable efficient recordings, micronail-type probes will be integrated with a CMOS readout circuitry. These micronails are structured to operate either as intracellular microelectrodes or as intracellular micro-syringes allowing either chemical injection or to produce pressure or suction that will be used in additional to other procedures to penetrate the cell membrane. With appropriate bio-functionalization, the micro-nails inserted intracellularly will also serve as sensors for phosphorylation/ dephosphorylation reactions within living cells.<br/>The main technologies and methods to be developed in the BRAINSTORM project include:<br/>\tBiocompatible and CMOS compatible micronails and micro-syringes with integrated readout electronics;<br/>\tMicrofluidic system integrated with the CMOS-containing chip;<br/>\tProcedures to insert both nail types through the plasma membrane, into the neuronal cytosole;<br/>\tSurface chemistries allowing to achieve a Giga-Ohm (G) seal resistance between the disrupted plasma membrane and the nail;<br/>\tHigh signal-to-noise ratio recordings of neuronal electrical activity with micronail probes;<br/>\tOn-chip chemical delivery of chemicals;<br/>\tOn-chip sensing of intracellular kinase activities.<br/>