REDBAC
Financiado
Occurrence, Regulation, and Function of Bacterial mRNA Editing Under Diverse Env...
"Bacteria are haploid, having a single copy of each gene. We suggest a paradigm shift in bacterial genetics: bacteria can have two RNA versions of the same gene, encoding protein isoforms at the single-bacterium and population lev...
"Bacteria are haploid, having a single copy of each gene. We suggest a paradigm shift in bacterial genetics: bacteria can have two RNA versions of the same gene, encoding protein isoforms at the single-bacterium and population level. These protein isoforms can have altered activity, which could be advantageous under different conditions.
Adenosine-to-inosine (A-to-I) mRNA editing can affect the sequence and the function of translated proteins because the ribosome identifies inosine as guanosine. We discovered that A-to-I mRNA editing occurs in bacteria (Escherichia coli), identified the mediating enzyme, and showed that it could affect protein function. Moreover, we discovered that environmental cues experienced during growth in culture affect bacterial mRNA editing. However, the prevalence and regulation of A-to-I mRNA editing across bacteria and the importance of editing for protein function and bacterial physiology in different environments are still unknown.
Here, we will test the hypothesis that mRNA editing is widespread in bacteria, producing two RNA ""alleles"" (pseudo-heterozygosity). Subsequently, these RNA ""alleles"" may co-exist in the same bacterial cell or population of cells. Thus, mRNA editing may enable the translation of protein isoforms, making bacteria better adapted to changing environments. We suggest (1) identifying mRNA editing events across environmental conditions in hundreds of species, (2) uncovering the regulatory genetic factors governing bacterial mRNA editing occurrence, and (3) determining the effect of mRNA editing on the proteome and its functional role in bacteria.
Our work could shed light on a new mechanism bacteria employ to grow and survive in different environments. Furthermore, bacterial mRNA editing—invisible to DNA sequencing—may account for unsolved problems or phenomena that current genetic and proteomic data cannot explain, affecting bacterial biology and human health."
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Duración del proyecto: 60 meses
Fecha Inicio: 2023-08-14
Fecha Fin: 2028-08-31
Fecha Fin: 2028-08-31
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-08-14
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
ERC-2023-STG:
ERC STARTING GRANTS
Cerrada
hace 2 años
Presupuesto
El presupuesto total del proyecto asciende a
2M€
Líder del proyecto
BENGURION UNIVERSITY OF THE NEGEV
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5