Innovating Works

ciTTub

Financiado
Molecular Basis of Tubulin Transport During Cilium Formation
Cilia are essential hair-like structures presented at the surface of eukaryotic cells that allow motility, fluid flow and complex inter- and intracellular signalling events. Nine pairs of microtubules organized in a cylindrical sh... Cilia are essential hair-like structures presented at the surface of eukaryotic cells that allow motility, fluid flow and complex inter- and intracellular signalling events. Nine pairs of microtubules organized in a cylindrical shape called axoneme form the backbone of cilia. The formation and maintenance of cilia is dependent on a multi-subunit protein complex termed the intraflagellar transport (IFT) complex that actively delivers axonemal building blocks such as tubulin from the base to the tip of growing cilia. Lack of cilia or its miss-construction leads to severe developmental diseases called ciliopathies. Despite its fundamental role in cilium biogenesis, the process of tubulin recruitment, loading and unloading by the IFT machinery remains poorly understood. In this proposal, I aim to elucidate the mechanism of tubulin loading onto IFT complexes by determining high-resolution structures of intraflagellar transport (IFT) complexes bound to tubulin. Details about the IFT-tubulin interaction interface will be obtained by a combination of biochemical techniques like site directed photo- and chemical crosslinking followed by mass-spectrometry analysis and the structural biology techniques X-ray crystallography and single particle cryo-electron microscopy (cryo-EM). Ultimately, this study will enrich our understanding of cilium biogenesis and homeostasis by providing the first insight at atomic resolution into cargo selection and loading onto IFT machinery. ver más
07/08/2022
AU
219K€
Perfil tecnológico estimado
Duración del proyecto: 29 meses Fecha Inicio: 2020-03-05
Fecha Fin: 2022-08-07

Línea de financiación: concedida

El organismo H2020 notifico la concesión del proyecto el día 2022-08-07
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
MSCA-IF-2019: Individual Fellowships
Cerrada hace 5 años
Presupuesto El presupuesto total del proyecto asciende a 219K€
Líder del proyecto
AARHUS UNIVERSITET No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5