Miniaturised Metabolomics Platform for Microvascular Research
Preventing and treating progressive microvascular loss (i.e. rarefaction) is a major challenge in cardiovascular medicine. Microvascular disease is a multifactorial disease with no effective treatment. Long-term exposure to advers...
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Información proyecto METAFORA
Duración del proyecto: 30 meses
Fecha Inicio: 2016-03-09
Fecha Fin: 2018-09-30
Líder del proyecto
UNIVERSITEIT LEIDEN
No se ha especificado una descripción o un objeto social para esta compañía.
TRL
4-5
Presupuesto del proyecto
178K€
Fecha límite de participación
Sin fecha límite de participación.
Descripción del proyecto
Preventing and treating progressive microvascular loss (i.e. rarefaction) is a major challenge in cardiovascular medicine. Microvascular disease is a multifactorial disease with no effective treatment. Long-term exposure to adverse metabolic and haemodynamic conditions such as hypertension, obesity and diabetes can cause microvascular destabilisation, loss of tissue capillaries, and eventual organ failure. Interestingly, not all diabetic patients develop kidney failure. The missing link to developing effective strategies for treating multifactorial diseases, including microvascular disease, is detailed mechanistic insight into the relationship between disease pathogenesis at the organ/cellular level at the systemic/organism level.
This project, METAFORA, aims to develop novel and analytical technologies to allow a new approach to understand the pathology of microvascular disease. METAFORA will develop a miniaturized metabolomics workflow to study metabolic pathways in an in-vitro microfluidic 3D microvasculature platform with organotypic functionality (the so-called organ-on-a-chip) to create personalised in-vitro models based on patient-derived human cells. This platform will enable the assessment of biochemical disease processes at the cell/organ level in the ‘whole-patient’ context by perfusing the patient’s own blood through the vasculature model.
METAFORA will use and optimize a novel separation and preconcentration method, depletion zone isotachophoresis, for the miniaturised analytical platform, which will be combined with mass spectrometry for metabolite profiling.