Mimicking Bacterial Middle Ear Infection in the Lab
Bacterial infections in the human body mostly involve biofilms - locally dense populations of bacteria that are extremely difficult to treat with antibiotics. Understanding antibiotic-biofilm interactions is crucial if we are to d...
Bacterial infections in the human body mostly involve biofilms - locally dense populations of bacteria that are extremely difficult to treat with antibiotics. Understanding antibiotic-biofilm interactions is crucial if we are to design better antibiotic treatment regimes to avoid the emergence of antibiotic resistance. In biofilms, bacteria are surrounded by a biopolymer matrix which can inhibit the motion of antibiotic molecules, leading to complex diffusive behaviour. Yet it is not known how these biophysical matrix-antibiotic interactions influence bacterial killing in a spatially complex infection model. Answering this question is the objective of my proposal. I will set up a lab model that mimics Acute Otitis Media -bacterial infection of the middle ear- and I will use advanced microscopy methods to track antibiotic molecules as they interact with bacterial biofilms. I will answer the following questions: A) what is the spatio-temporal distribution of antibiotic molecules in the biofilm? and B) What is the effect of biofilm structure on the antibiotic response, at the single-cell level? After establishing materials and protocols (objective 1), I will use fluorescence-correlation-spectroscopy (FCS) to characterize the spatial distribution of antibiotic molecules (objective 2). Next, I will use state-of-art 2D STED-FCS method (combination of FCS with stimulated-emission depletion microscopy) to track in unprecedented detail how antibiotics kill individual bacteria within a biofilm (objective 3). Finally, by tracking bacterial growth over long times, I will determine how antibiotic resistance emerges in these spatially complex biofilms (objective 4). I will be supported by the world-class expertise of the host, Prof. Christian Eggeling (Leibniz IPHT Jena, Germany), who has pioneered the advanced super-resolution microscopy methods that I will use, and by an interdisciplinary team of clinical, chemical and microbiological collaborators in Jena.ver más
Seleccionando "Aceptar todas las cookies" acepta el uso de cookies para ayudarnos a brindarle una mejor experiencia de usuario y para analizar el uso del sitio web. Al hacer clic en "Ajustar tus preferencias" puede elegir qué cookies permitir. Solo las cookies esenciales son necesarias para el correcto funcionamiento de nuestro sitio web y no se pueden rechazar.
Cookie settings
Nuestro sitio web almacena cuatro tipos de cookies. En cualquier momento puede elegir qué cookies acepta y cuáles rechaza. Puede obtener más información sobre qué son las cookies y qué tipos de cookies almacenamos en nuestra Política de cookies.
Son necesarias por razones técnicas. Sin ellas, este sitio web podría no funcionar correctamente.
Son necesarias para una funcionalidad específica en el sitio web. Sin ellos, algunas características pueden estar deshabilitadas.
Nos permite analizar el uso del sitio web y mejorar la experiencia del visitante.
Nos permite personalizar su experiencia y enviarle contenido y ofertas relevantes, en este sitio web y en otros sitios web.