Innovating Works

NaNoLens

Financiado
Lensless label-free nanoscopy
Optical nanoscopy has changed the “seeing is believing” paradigm. This was achieved within a limited field of view (FOV~100µm2) and required fluorescent markers. Large-FOV high-throughput live unimpaired cell imaging is crucial fo... Optical nanoscopy has changed the “seeing is believing” paradigm. This was achieved within a limited field of view (FOV~100µm2) and required fluorescent markers. Large-FOV high-throughput live unimpaired cell imaging is crucial for biology and biomedicine. Hence, improving the space bandwidth product (SBP) using time-consuming scanning stitching is not a good solution. Lensless holographic microscopy (LHM) inherently bypasses FOV limitations by using full sensor-size hologram reconstruction for label-free object information retrieval. Its major limitation, not yet addressed, is its low lateral (~1µm) and axial (~3µm) resolution. I will overcome this fundamental problem by pioneering deep UV (DUV) lensless holotomographic nanoscopy (LHN) as a simple and compact device easily operated inside the cell chamber or outside the laboratory (in contrast to lens-based systems). Owing to DNA damage, DUV is used to sterilize and never image bio-samples. This paradigm will be shifted to provide a breakthrough 10 giga pixel SBP via a low-dose DUV optical-elements-free (no cost, no radiation loss) lensless setup with a worlds-first full-angle tomographic scenario, numerical aperture > 1, and a new class of reconstruction algorithms to decrease the effective pixel (to 100 nm) and remove background noise. I will use LHN to enable the discovery of a new mechanistic understanding of extracellular vesicles expression and intake within large live cell cultures with single-vesicle resolution. EVs, nanosized lipid spheres released by virtually every cell type, are currently emerging as novel disease biomarkers and drug nanovehicles. LHN is a new research field that inherently makes this a high-risk project, but the potential gains are also high as a new era of simple ultrahigh SBP nanoimaging might be opened. The proposed multidisciplinary project calls for near-unique expertise in computational microscopy and digital holography, which I acquired in cooperation with international leaders. ver más
31/12/2028
2M€
Duración del proyecto: 59 meses Fecha Inicio: 2024-01-01
Fecha Fin: 2028-12-31

Línea de financiación: concedida

El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2024-01-01
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
ERC-2023-STG: ERC STARTING GRANTS
Cerrada hace 2 años
Presupuesto El presupuesto total del proyecto asciende a 2M€
Líder del proyecto
POLITECHNIKA WARSZAWSKA No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5