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Harvesting Light for Life: Green Proteins as the Interface between Sun Energy an...

Harvesting Light for Life: Green Proteins as the Interface between Sun Energy and Biosphere Life on earth feeds on photons. Photosynthesis in green algae and land plants has been the world’s most successful biological process and has conquered the most diverse environments. Photosynthetic reaction centres are extremely w... ver más

30/09/2027

UNIVR

2M€

Presupuesto del proyecto: 2M€

Líder del proyecto

UNIVERSITA DEGLI STUDI DI VERONA No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2022-10-01

ERC-2021-ADG: ERC ADVANCED GRANTS Scope:Objectives

I+D

Cerrada hace 3 años

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Este proyecto no cuenta con búsquedas de partenariado abiertas en este momento.

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UNIVR

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Duración del proyecto: 59 meses Fecha Inicio: 2022-10-01
Fecha Fin: 2027-09-30

Líder del proyecto

UNIVERSITA DEGLI STUDI DI VERONA No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 2M€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto Life on earth feeds on photons. Photosynthesis in green algae and land plants has been the world’s most successful biological process and has conquered the most diverse environments. Photosynthetic reaction centres are extremely well conserved, an unlikely basis for the ability to adapt. Antenna systems are widely diversified and yet only the Light-Harvesting Complexes (LHCs) have been selected for growth in the land environment. The distinctive property of GreenCut organisms lies in their light-harvesting mechanisms, which ensure efficient photon harvesting and photoprotection. Despite being the most abundant membrane proteins on earth, binding most of chlorophyll that makes the planet green, the secrets of LHCs are still concealed because we lack experimental systems that make possible the reverse and forward genetic analysis of LHCII proteins. Indeed, the clustered-genes encoding LHCII has resisted targeting by classic genetics. I propose an in-depth analysis of LHCII proteins in algae and plants by deploying a new technology that we have developed with our experience in studying LHCs. Firstly, we deleted all genes-encoding LHCII in model species of both land plants and green algae by genome editing and complemented plant ΔLHCII lines with site-directed, mutated sequences, demonstrating that reverse genetics can reveal the domains involved in the regulation of photon harvesting, photoprotection and growth. Secondly, forward genetics, on the other hand, will enable the identification of protein determinants by selecting specific phenotypes on complementing mixotrophic algal ΔLHCII lines with randomly mutagenized sequences. This will lead to a map of structures and functions that identifies the specific biological role of each component of the antenna system in vivo and in vitro. The project’s outcome will be the ability to design in a rational way the light-harvesting systems of plants and algae in the context of sustainable agriculture and bio-industry.

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