Flavin-containing monooxygenases (FMOs) are a highly conserved family of enzymes present in prokaryotes, fungi, animals, and plants. Among plant FMOs, the N-hydroxylating FMO1 is essential for pathogen resistance. FMO1 catalyses t...
ver más
¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video
Proyectos interesantes
AGL2016-76216-C2-1-R
DESARROLLO DE NUEVAS HERRAMIENTAS PARA EL CONTROL DE OIDIOS
224K€
Cerrado
RYC-2010-05759
Metabolic flux analysis and engineering of plant isoprenoids...
192K€
Cerrado
PID2021-124813OB-C32
IDENTIFICANDO PATRONES COMUNES DE PRIMING DURANTE LA INDUCCI...
218K€
Cerrado
AGL2016-76216-C2-2-R
DESARROLLO DE NUEVAS HERRAMIENTAS PARA EL CONTROL DE OIDIOS
103K€
Cerrado
BIO2012-32838
FUNCION REGULADORA DE MICRORNAS EN LA RESPUESTA INMUNE DE LA...
269K€
Cerrado
AGL2012-35539
GENETICA Y MEJORA DE BRASICAS HORTICOLAS: PAPEL DE LOS METAB...
234K€
Cerrado
Información proyecto MultiPly
Duración del proyecto: 29 meses
Fecha Inicio: 2023-03-16
Fecha Fin: 2025-08-31
Líder del proyecto
KOBENHAVNS UNIVERSITET
No se ha especificado una descripción o un objeto social para esta compañía.
TRL
4-5
Presupuesto del proyecto
231K€
Fecha límite de participación
Sin fecha límite de participación.
Descripción del proyecto
Flavin-containing monooxygenases (FMOs) are a highly conserved family of enzymes present in prokaryotes, fungi, animals, and plants. Among plant FMOs, the N-hydroxylating FMO1 is essential for pathogen resistance. FMO1 catalyses the biosynthetic reaction to synthesize N-hydroxy-pipecolic acid (N-Pip), the critical signalling molecule for Systemic Acquired Resistance (SAR) defense priming. Since the identification of FMO1 functionality in 2018, targeted analysis of the N-Pip pathway shows high potential for metabolic engineering to enhance disease resistance in dicotyledons. A fully analogous role for N-Pip SAR in monocotyledonous crops, however, is less clear. In fact, preliminary analysis by the Neilson Group (UCPH) suggests the FMO1 from barley (Hordeum vulgare) and sorghum (Sorghum bicolor) has diversified functionality compared to activity observed in the FMO1 from Arabidopsis. Unbiased analysis of potential FMO1 multifunctionality has not yet been addressed, and detailed analysis of FMO1 evolution throughout the plant kingdom is lacking. The proposed project – ‘MultiPly: FMO1 Multifunctionality for improved Plant health’–will employ an interdisciplinary approach to investigate the evolution and functionality of the significant Plant FMO1s. Specifically, this project will combine bioinformatics (phylogeny reconstruction, protein modelling, and docking) with enzyme kinetic studies and in planta analysis to assess FMO1 multifunctionality throughout the plant kingdom. The in planta FMO1 characterization will primarily occur in barley to unravel the mode of SAR in these agriculturally important crops. In addition, MultiPly is designed to utilize my background in protein biochemistry and bioinformatics. I will also receive significant training within the new technical fields of metabolomics and plant physiology, as well as scientific management, leadership, and mentorship to establish an independent research group in academia.